"The project is focussed on feasibility study of recombinant expression of CYP3A4 enzyme for in virto tests of drug biotransformation. The project is based on cloning gene for CYP3A4 into baculovirus expression vector and succesive production of the enzyme in insect host cells in vitro."@en . "0"^^ . "In vitro expression of recombinant protein CYP3A4 for study of metabolic changes of drugs:feasibility study"@en . "1"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NL6177"^^ . . "Projekt je zam\u011B\u0159en na studium proveditelnosti rekombinantn\u00ED syst\u00E9my enzymu CYP3A4 pro pou\u017Eit\u00ED k in vitro studiu biotransformace l\u00E9\u010Div. Projekt je zalo\u017Een na inkorporaci genu pro CYP3A4 do bakulovirov\u00E9ho expresn\u00EDho vektoru a produkci enzymu ve hmyz\u00EDch hostitelsk\u00FDch bu\u0148k\u00E1ch in vitro." . "C\u00EDlem projektu bylo vypracovat metodu rekombinantn\u00ED synt\u00E9zy cytochromu P450 3A4, jeho inkorporace do vektoru a\u017E po n\u00E1slednou produkci enzymu v hmyz\u00EDch bu\u0148k\u00E1ch. Zvl\u00E1dnut\u00ED modern\u00EDch metod."@cs . "NL6177" . . "vector" . . . "CYP3A4" . "CYP3A4;vector;feasibility;recombinant;baculovirus;biotransformation;molecular;drug"@en . "biotransformation" . . "recombinant" . . "0"^^ . "feasibility" . . "In vitro exprese rekombinantn\u00EDho proteinu CYP3A4 pro studium metabolick\u00FDch p\u0159em\u011Bn l\u00E9\u010Div: studium proveditelnosti" . "2003-07-31+02:00"^^ . . "baculovirus" . . . "molecular" . . . "1"^^ . . . . . "1"^^ .