. "Cytochrome P450;Reductase;Anticancer;Antioxidants;Radicalis;Peroxidation;Cancer"@en . . . . "http://www.isvav.cz/projectDetail.do?rowId=NL6095"^^ . "Anticancer" . "Antioxidants" . "C\u00EDlem je odhalit \u00FAlohu jednotliv\u00FDch slo\u017Eek monooxygen\u00E1zov\u00E9ho syst\u00E9mu ve vzniku oxidativn\u00EDho stresu a toxicity chinon\u016F. Za t\u00EDmto \u00FA\u010Delem bude analyzov\u00E1na tvorba reaktivn\u00EDch forem kysl\u00EDku,organick\u00FDch radik\u00E1l\u016F,peroxidace lipid\u016F a po\u0161kozen\u00ED aktivity cytochrom\u016F P450 v kask\u00E1d\u011B syst\u00E9m\u016F: rekonstituovan\u00FD monooxygen\u00E1zov\u00FD-subcelul\u00E1rn\u00ED (mikrosomy)-bun\u011B\u010Dn\u00E9 linie. Analyzov\u00E1no bude p\u016Fsoben\u00ED modelov\u00FDch l\u00E1tek(katechol,hydrochinon a benzochinon) a chinonick\u00FDch cytostatik (antracykliny).Dal\u0161\u00EDm c\u00EDlem bude nal\u00E9zt l\u00E1tky a faktory,kter\u00E9 by sn\u00ED\u017Eily cytotoxicitu chinon\u016F(antioxidanty,lapa\u010De radik\u00E1l\u016F,chel\u00E1tory)." . "Study of the role of biotransformation enzymes in chemical carcinigenesis"@en . . "Cytochrome P450" . . . "Studium \u00FAlohy bitransforma\u010Dn\u00EDch enzym\u016F v chemick\u00E9 karcinogenezi" . "Radicalis" . . . . . . "0"^^ . "Auto\u0159i systematicky propracovali rozd\u00EDly v antioxida\u010Dn\u00ED aktivit\u011B polyfenol\u016F a p\u0159isp\u011Bli k rozpozn\u00E1n\u00ED odolnosti jednotliv\u00FDch cytochrom\u016F P450 k oxida\u010Dn\u00EDmu po\u0161kozen\u00ED."@cs . . "2003-07-31+02:00"^^ . "The aim of the projects is to evaluate the role of individual components of monooxygenase system in the induction of oxidative stress and toxicity of quinones. Presence of reactive oxyden species,organic radicals,peroxidation of lipids,and cytochrome P450 destruction will be analyzed in the cascade of systems:model monooxygenase system-subcellular fractions-cell lines. The effects of model chemicals (catechol,hydroquinone,benzoquinone)and chinonic anticancer drugs (anthracyclines)will be studied and protective factors (antioxidants,radical acavengers,chelators)will be employed in order to decrease quinone cytotoxicity."@en . . . "NL6095" . "3"^^ . "0"^^ . "Peroxidation" . "3"^^ . "Reductase" . . "1"^^ .