. . "DNA" . . "Objasn\u011Bn\u00ED genotoxick\u00FDch \u00FA\u010Dink\u016F r\u016Fzn\u011B prostorov\u011B uspo\u0159\u00E1dan\u00FDch deriv\u00E1t\u016F platiny (trans-EE a cis-platiny) u Escherichia coli. V\u00FDznamn\u00FD poznatek, \u017Ee L-methionin inhibuje reakci DNA s cis-diaminodichlorplatnat\u00FDm komplexem."@cs . "1"^^ . . . "1"^^ . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=NL6069"^^ . "Mutagenicity" . . "5"^^ . "Projekt je zam\u011B\u0159en na vytvo\u0159en\u00ED teoretick\u00E9ho z\u00E1zem\u00ED nutn\u00E9ho pro rozvoj nov\u00FDch protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDch l\u00E9\u010Div odvozen\u00FDch od komponent\u016F platiny.C\u00EDlem je vyhodnotit rizika plynouc\u00ED z mutagenn\u00EDch \u00FA\u010Dink\u016F nov\u00FDch platinov\u00FDch cytostatik. Mutagenn\u00ED \u00FA\u010Dinky nov\u00FDch cytostatik budou hodnoceny Amesov\u00FDmi testy a pomoc\u00ED sekvena\u010Dn\u00ED anal\u00FDzy DNA syntetizovan\u00E9 na templ\u00E1tech nesouc\u00EDch specifick\u00E9 adukty komponent\u016F platiny. Zm\u011Bny v konformaci DNA vyvolan\u00E9 adukty protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDch komplex\u016F platiny budou zji\u0161t\u011Bny pomoc\u00ED modern\u00EDch metod molekul\u00E1rn\u00ED biologie a bifyziky.Zji\u0161t\u011Bn\u00E9 zm\u011Bny budou uvedeny do vztahu k mutagenn\u00ED \u00FA\u010Dinnosti t\u011Bchto cytostatik. Projekt p\u0159isp\u011Bje k \u0159e\u0161en\u00ED probl\u00E9m\u016F zdravotnictv\u00ED,souvisej\u00EDc\u00EDch s v\u00FDzkumem nov\u00FDch l\u00E1tek pro l\u00E9ka\u0159sk\u00E9 \u00FA\u010Dely (cytostatik),objas\u0148ov\u00E1n\u00ED jejich ne\u017E\u00E1douc\u00EDch \u00FA\u010Dink\u016F a bezpe\u010Dnosti." . "5"^^ . "cancer" . "platinum" . . . "Mutagenn\u00ED efekty cytostatik odvozen\u00FDch od komponent\u016F platiny. Vztah k v\u00FDvoji nov\u00FDch l\u00E9\u010Div proti rakovin\u011B" . . "2003-07-31+02:00"^^ . "The project is aimed at developing a theoretical background for systhesis and development of antitumor platinum and ruthenium drugs.The main goal is to evaluate the risk associated with mutagenic effects of novel platinum antitumor drugs. Mutagenic effects of new antitumor drugs woll be evaluated by Ames tests and with the aid of direct sequencing of DNA synthesized on templates containing sitespecifically placed platinum DNA adducts. Alterations in DNA conformation induced by the adducts of antitumor platinum drugs will be determined with aid of moderm methods of molecular biology and biophysics.These alterations will be to mutagenic efficiency of these drugs. The project will conrtibute to solving the problems of health care associated with the for new antitumor drugs,clarification of rheir side effects and safety."@en . "0"^^ . . "Mutegenic effects of platinum compounds.Relations to the development of new anticancer drugs"@en . . . "NL6069" . "Mutagenicity;DNA;platinum;cancer;antitumor drugs"@en . .