. "HUMARA" . "Vy\u0161et\u0159en\u00ED klonality pomoc\u00ED XCIP prok\u00E1zalo v\u00FDznamn\u00FD rozd\u00EDl v incidenci klon\u00E1l.subpopul.mezi nemocn\u00FDmi s RA a RCMD podtypy MDS,anal\u00FDza klinick.parametr\u016F potvrdila nep\u0159\u00EDznivou progn\u00F3zu nemocn\u00FDch s RCMD oproti RA."@cs . . "2006-06-23+02:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=NK7713"^^ . . . "Investigation of clonality in granulocytes CD 14+ monocytes and CD+ lympocytes isolated from bone marrow of patients with different stages of myelodysplastic syndrome using detection of X- chromosome inactivation pattern with HUMARA. Determination of the role of clonality detection in MDS patients by correlation of the results of clonality assay with karyotype investigation using FISH, with the rate of apoptosis of cell subpopulations and with CFU-GM in vitro growth. Analysis of repeated clonality investigation in the course of the disease for diagnosis, prognosis and treatment of MDS and evaluation of clonality as an early marker of disease progression, analysis of possible clonal proliferation of T lymphocyte subp."@en . "apoptosis" . "Detection of clonality using XCIP revealed a significant difference in incidence of clonal cell subpopul.between RA and RCMD MDS subtypes,analysis of clinical parameters confirmed adverse prognosis of RCMD compared to RA."@en . "clonality" . . . "myelodysplasia;clonality;HUMARA;FISH;apoptosis;bone marrow cultures;prognosis;treatment"@en . . . "0"^^ . . . . "1"^^ . "FISH" . "0"^^ . "myelodysplasia" . "prognosis" . "NK7713" . "11"^^ . "11"^^ . . . . . "The role of detection of clonality in diagnosis, prognosis and treatment of myelodysplatic syndrome"@en . "Studium klonality na isolovan\u00FDch subpopulac\u00EDch granulocyt\u016F, CD14 positivn\u00EDch monocyt\u016F a CD3 positivn\u00EDch lymfocyt\u016F isolovan\u00FDch z kostn\u00ED d\u0159en\u011B nemocn\u00FDch s r\u016Fzn\u00FDmi formami myelodysplastick\u00E9ho syndromu metodikou polymorfismu d\u00E9lky restrik\u010Dn\u00EDch fragment\u016F genu Ur\u010Den\u00ED role vy\u0161et\u0159en\u00ED klonality u nemocn\u00FDch s myelodysplastick\u00FDm syndromem korelac\u00ED v\u00FDsledk\u016F vy\u0161et\u0159en\u00ED s cytogenetick\u00FDmi n\u00E1lezy metodou FISH, se stupn\u011Bm apoptosy jednotliv\u00FDch bun\u011B\u010Dn\u00FDch subpopulac\u00ED a s n\u00E1lezy kultivace d\u0159e\u0148ov\u00FDch bun\u011Bk in vitro. Posouzen\u00ED v\u00FDznamu vy\u0161et\u0159en\u00ED klonality v pr\u016Fb\u011Bhu choroby pro diagnosu, prognosu a l\u00E9\u010Debn\u00E9 ovlivn\u011Bn\u00ED choroby, zejm\u00E9na jako \u010Dasn\u00E9ho ukazatele progrese onemocn\u011Bn\u00ED. Sledov\u00E1n\u00ED klon\u00E1ln\u00EDho charakteru subpopulace T mylfocyt\u016F ve vztahu k racion\u00E1ln\u00ED indikaci imunos" . . "V\u00FDznam vy\u0161et\u0159en\u00ED klonality pro diagnosu, prognosu a l\u00E9\u010Debn\u00E9 ovlivn\u011Bn\u00ED myelodysplastick\u00E9ho syndromu" . . "bone marrow cultures" . . .