"gene expression" . "2006-06-23+02:00"^^ . . "biopolymeric scaffolds" . . . . "6"^^ . "orthopedics" . . "mesenchymal stem cells;orthopedics;nonuniting fractures;gene expression;biopolymeric scaffolds;NOD/SCID mice"@en . "6"^^ . "mesenchymal stem cells" . "ND7448" . "Vyu\u017Eit\u00ED autologn\u00EDch mezenchymov\u00FDch bun\u011Bk v rekonstruk\u010Dn\u00ED ortopedii" . "Mesenchymal stem cells from patients undergoing routine bone marrow examination will be cultived in vitro in media with fetal calf serum and human adult and cord blood serum and differentiated toward osteoblastic lineage. Gene experssion of these ostaoblasts will be compared with gene expression of osteoblastes from bone obtained after orthopedics research surgery. Osteoblasts multiplied in vitro will be implanted in NOD/SCID mice on biodegradable scaffolds. Behaviour of the cells and scaffolds in NOD/SCID mice will be studied to evaluate the growth on the cells, bone matrix formation and local and systemic adverse affects. Clinical part of the study will involve injection of multiplied, predifferentiated autologous mesenchymal cells grown in media without fetal serum to patients with nonuniting fractures."@en . . . "Use of mesenchymal stem cells in reconstruction orthopaedics surgery"@en . . . . . "http://www.isvav.cz/projectDetail.do?rowId=ND7448"^^ . "Mezenchymov\u00E9 progenitorov\u00E9 bu\u0148ky od pacient\u016F podstupuj\u00EDc\u00EDch be\u017En\u00E9 vy\u0161et\u0159en\u00ED kostn\u00ED d\u0159en\u011B budou kultivov\u00E1ny in vitro v m\u00E9di\u00EDch s fet\u00E1ln\u00EDm telec\u00EDm s\u00E9rem, lidsk\u00FDm s\u00E9rem skupiny AB a umbilik\u00E1ln\u00EDm lidsk\u00FDm s\u00E9rem a diferencov\u00E1ny sm\u011Brem k osteoblastick\u00E9 linii. Genov\u00E1 exprese takto z\u00EDskan\u00FDch bun\u011Bk bude srovn\u00E1na s genovou expres\u00ED lidsk\u00FDch osteoblast\u016F, z\u00EDskan\u00FDch p\u0159i ortopedick\u00FDch resek\u010Dn\u00EDch v\u00FDkonech. Bu\u0148ky namno\u017Een\u00E9 in vitro budou d\u00E1le aplikov\u00E1ny pod k\u016F\u017Ei imunodeficientn\u00EDch my\u0161\u00ED na biodegradabiln\u00EDm nosi\u010Di. Sledov\u00E1no bude chov\u00E1n\u00ED bun\u011Bk a biodegradabiln\u00EDho nosi\u010De v organismu imunodeficientn\u00EDch my\u0161\u00ED, m\u00EDstn\u00ED a celkov\u00E9 ne\u017E\u00E1douc\u00ED \u00FA\u010Dinky. Klinick\u00E1 \u010D\u00E1st studie po\u010D\u00EDt\u00E1, po spln\u011Bn\u00ED po\u017Eadavk\u016F S\u00DAKL na dobrou klinickou praxi, s mno\u017Een\u00EDm autologn\u00EDch mezenchymov\u00FDch bun\u011Bk in vitro bez pou\u017Eit\u00ED zv\u00ED\u0159ec\u00EDho s\u00E9re a s jejich injek\u010Dn\u00ED aplikac\u00ED do m\u00EDsta nehoj\u00EDc\u00EDch se fraktur u ortopedick\u00FDch pacient\u016F." . . . . "V\u00FDsledkem \u0159e\u0161en\u00ED je patentov\u00E1 p\u0159ihl\u00E1\u0161ka s n\u00E1zvem Zp\u016Fsob p\u011Bstov\u00E1n\u00ED lidsk\u00FDch mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk,zejm\u00E9na pro l\u00E9\u010Dbu nehoj\u00EDc\u00EDch se fraktur, a bioreaktor k prov\u00E1d\u011Bn\u00ED tohoto zp\u016Fsobu."@cs . . . "The result of the project is the patent application with title Method of cultivation of human mesenchymal stem cells,especially for the purpose of treatment of nonunion fracturesa and bioreactor for realization of this method."@en . "nonuniting fractures" . "1"^^ . . "2"^^ . . . . "0"^^ .