"Kultivace a expanze hematopoetick\u00FDch kmenov\u00FDch bun\u011Bk in vitro - optimalizace selekce bun\u011Bk a kultiva\u010Dn\u00EDch podm\u00EDnek." . . "immunomagnetic separation" . . . . "To optimize immunomagnetic selection of hematopoietic stem cells, for maximum achievable purity, yield. and recovery parameters. To find the best combination of cytokines which would allow maximum expansion of granulocytic progenitor cells in the ex vivo culture. To verify the possibility of expansion of megakaryocytic progenitor cells in ex vivo culture in the presence of specific cytokines. To evaluate results by morphology, immunophenotyping, and cultivation of CFU-GM and CFU-Meg colonies."@en . "cytokines" . "1"^^ . . "CFU-GM" . "0"^^ . "Pou\u017Eit\u00ED dvou metod imunomagnetick\u00E9 selekce CD34+ bun\u011Bk."@cs . "2"^^ . "2"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=NC7493"^^ . . . "0"^^ . . "2004-07-12+02:00"^^ . "NC7493" . . . . "hematopoietic stem cells" . . . . "Optimalizovat omunogemagnetick\u00FD syst\u00E9m selekce hematopoetick\u00FDch bun\u011Bk s c\u00EDlem maxim\u00E1ln\u00ED v\u00FDt\u011B\u017Enosti a \u010Distoty bun\u011B\u010Dn\u00E9 frakce - srovn\u00E1n\u00ED metody positivn\u00ED selekce CD34+ bun\u011Bk a negativn\u00ED selekce Lin-bun\u011Bk. Identifikovat nejvhodn\u011Bj\u0161\u00ED slo\u017Een\u00ED cytokinov\u00E9 sm\u011Bsi, je\u017E umo\u017En\u00ED maxim\u00E1ln\u00ED expanzi granulocyt\u00E1rn\u00EDch progenitorov\u00FDch bun\u011Bk v in vitro kultu\u0159e v serum-free a stroma-free mediu. Iv\u011B\u0159it mo\u017Enost expanze megakaryocyt\u00E1rn\u00EDch progenitor\u016F v in vitro kultu\u0159e v p\u0159\u00EDtomnosti specifick\u00FDch cytokin\u016F-progenitory budou analyzov\u00E1ny technikou kultivace CFU-Meg. Zhodnotit a srovnat v\u00FDsledky ex vivo expanz\u00ED pomoc\u00ED imunofenotypizace, morfologick\u00E9 anal\u00FDzy a p\u0159\u00EDmou kultivac\u00ED progemitor\u016F CFU-GM a CFU-Meg." . "in vitro culture" . . "hematopoietic stem cells;immunomagnetic separation;cytokines;in vitro culture;CFU-GM;immunophenotyping"@en . "Cultivation and expansion of hematopoietic stem cells in vitro - which cell selection method and culture conditions should be used?"@en .