"V\u00FDvoj a pou\u017Eit\u00ED monoklon\u00E1ln\u00EDch protil\u00E1tek pro detekci abnorm\u00E1ln\u00ED a bun\u011B\u010Dn\u00E9 formy prionov\u00E9ho proteinu (PrPtse/PrPc) v krvi a tk\u00E1\u0148ov\u00FDch kultur\u00E1ch" . . . "New developed antibodies against prion protein were utilized to reveal the presence of prion protein fragment PrP226* in the brain tissue and for the development of the PrP226* assay allowing diagnosis of patients with Creutzfedt-Jakob disease."@en . . . "Development and Utilization of Anti- Prion Monoclonal Antibodies for Detection of PrPtse/PrPc in Cell Cultures and Blood"@en . . "Prion protein \u2013 PrP; antiPrP monoclonal antibody; cell cultures; blood"@en . "1. Utilize different anti-PrP mAbs, developed by the Slovene partner for the detection of different strains of PrPtse in prion infected mouse cell cultures of neuronal or fibroblast origin in different stages of cell division provided by #ORIGch partner laboratory. 2. Testing of the ability of anti-PrP mAbs to abrogate accumulation of PrPtse in prion infected cell cultures. 3. Utilization of europium labeled anti-PrPtse fragment specific mAbV5B2, and other anti-PrP mAbs, developed in Curin Serbec\u2019s lab, (Serbec et al.J.Biol.Chem 2004, 279, 3694-98), for the development of a sensitive ELISA assay for detection of PrPtse in blood and cell lysates."@en . "1"^^ . "0"^^ . "1"^^ . . . . "1. Detekce r\u016Fzn\u00FDch kmen\u016F prion\u016F v my\u0161\u00EDch bun\u011B\u010Dn\u00FDch lini\u00EDch nervov\u00E9ho \u010Di fibroblastov\u00E9ho p\u016Fvodu za vyu\u017Eit\u00ED r\u016Fzn\u00FDch anti-PrP mAb, kter\u00E9 poskytne slovinsk\u00FD partner. 2. Testov\u00E1n\u00ED \u00FA\u010Dinnosti t\u011Bchto mAb p\u0159i odl\u00E9\u010Dov\u00E1n\u00ED prionov\u00E9 infekce v bun\u011B\u010Dn\u00FDch kultur\u00E1ch 3. V\u00FDvoj senzitivn\u00EDho testu na b\u00E1zi ELISA pro detekci PrPtse v krvi a bun\u011B\u010Dn\u00FDch kultur\u00E1ch s vyu\u017Eit\u00EDm fragmentu specifick\u00E9ho pro mAb-V5B2 zna\u010Den\u00E9ho Europiem." . "1"^^ . . . "2010-01-01+01:00"^^ . . "0"^^ . . "2011-12-31+01:00"^^ . "2012-07-12+02:00"^^ . . . . "2011-03-04+01:00"^^ . " antiPrP monoclonal antibody" . " cell cultures" . "http://www.isvav.cz/projectDetail.do?rowId=MEB091004"^^ . "MEB091004" . "Prion protein \u2013 PrP" . . . "Za pou\u017Eit\u00ED nov\u011B p\u0159ipraven\u00FDch protil\u00E1tek proti prionov\u00E9mu proteinu bylo zji\u0161t\u011Bno, \u017Ee mozkov\u00E1 tk\u00E1\u0148 obsahuje fragment prionov\u00E9ho proteinu PrP226* a byla vyvinuta metoda jeho stanoven\u00ED umo\u017E\u0148uj\u00EDc\u00ED diagnostiku pacient\u016F s\u00A0Creutzfedt-Jakobovou nemoc\u00ED."@cs . . .