"A number of studies, including our own preliminary data, have shown that TRAIL (an important member of the TNF family) works synergistically with some anticancer drugs to kill most types of tumor cells and reversed resistance to such drugs. These findings indicate that the combination of TRAIL and chemotherapeutic drugs is potentially promising in treating refractory cancers. TRAIL can induce human cancer cell apoptosis through engagement of its death receptors DR4 and DR5. However, not all cancers are sensitive to this cytokine. Recently, it was shown that low doses of cytotoxic drugs could restore TRAIL-induced cell death in resistant colon and prostate cancer cell lines. Taken together, finding mechanisms leading to sensitizing the cancer cells to chemotherapy represent a useful approach for identifying new and/or improving existing anti-cancer strategies. The general aims of the proposal are 1) To identify if and how the treatment of the cells by DHA modulate association of selected death recep"@en . "Role of lipid rafts in regulation of cell signalling leading to modulation of cytokinetics of cancer cells"@en . . . . . . "2008-08-13+02:00"^^ . "A number of studies, including our own preliminary data, have shown that TRAIL (an important member of the TNF family) works synergistically with some anticancer drugs to kill most types of tumor cells and reversed resistance to such drugs. These findings indicate that the combination of TRAIL and chemotherapeutic drugs is potentially promising in treating refractory cancers. TRAIL can induce human cancer cell apoptosis through engagement of its death receptors DR4 and DR5. However, not all cancers are sensitive to this cytokine. Recently, it was shown that low doses of cytotoxic drugs could restore TRAIL-induced cell death in resistant colon and prostate cancer cell lines. Taken together, finding mechanisms leading to sensitizing the cancer cells to chemotherapy represent a useful approach for identifying new and/or improving existing anti-cancer strategies. The general aims of the proposal are 1) To identify if and how the treatment of the cells by DHA modulate association of selected death recep" . "The main contribution was on the methodological level. The results demonstrated that Pt-cytostatics may modulate signalling of TRAIL by different mechanisms - changes of cell membrane, expression of receptors and kinetics of internalization of TRAIL."@en . . "0"^^ . "2008-01-01+01:00"^^ . . . "1"^^ . "2008-12-31+01:00"^^ . "0"^^ . "\u00DAloha lipidov\u00FDch raft\u016F v \u0159\u00EDzen\u00ED bun\u011B\u010Dn\u00E9 signalizace vedouc\u00ED k modulaci cytokinetiky n\u00E1dorov\u00FDch bun\u011Bk" . . "0"^^ . "Hlavn\u00ED p\u0159\u00EDnos byl v oblasti metodologick\u00E9. D\u00E1le v\u00FDsledky uk\u00E1zaly, \u017Ee Pt-cytostatika mohou modulovat sign\u00E1ln\u00ED dr\u00E1hu TRAIL pomoc\u00ED \u0159ady r\u016Fzn\u00FDch mechanism\u016F, kter\u00E9 zahrnuj\u00ED zm\u011Bny na \u00FArovni bun\u011B\u010Dn\u00E9 membr\u00E1ny, exprese receptor\u016F i kinetiky internalizace TRAIL."@cs . . "http://www.isvav.cz/projectDetail.do?rowId=MEB040703"^^ . "cancer- cell signalling- lipid rafts- TRAIL- cisplatin- colocalization- FRET- cytometry-"@en . "0"^^ . . . . . "2010-05-07+02:00"^^ . "MEB040703" . . . .