. "Invasive species" . "2012-02-15+01:00"^^ . . . "The structure and expression of ribosomal RNA genes in invasive Spartina anglica allopolyploid species that formed recently in Europe"@en . . . . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=MEB021114"^^ . "Invasive species; allopolyploid; rDNA; evolution; genome"@en . . . . . "2012-12-31+01:00"^^ . "C\u00EDlem projektu je studium struktury a exprese gen\u016F pro ribozom\u00E1ln\u00ED RNA v p\u0159irozen\u00FDch populac\u00EDch invazivn\u00EDho travn\u00EDho druhu Spartina anglica. 1. Genetick\u00E9 aspekty studia. V t\u00E9to \u010D\u00E1sti se budeme v\u011Bnovat ot\u00E1zk\u00E1m genetick\u00FDch interakc\u00ED, rekombinac\u00ED a homogenizac\u00ED rodi\u010Dovsk\u00FDch gen\u016F rRNA. Bude uskute\u010Dn\u011Bna genetick\u00E1 anal\u00FDza populac\u00ED poch\u00E1zej\u00EDc\u00EDch z r\u016Fzn\u00FDch lokalit francouzsk\u00E9ho a britsk\u00E9ho pob\u0159e\u017E\u00ED (viz. obr. 1). Budeme \u0159e\u0161it ot\u00E1zku, zda genetick\u00E9 zm\u011Bny v lokus rDNA (pokud budou zaznamen\u00E1ny) jsou vyvolan\u00E9 mezidruhovou hybridizac\u00ED nebo n\u00E1slednou polyploidi\u00ED. Experiment\u00E1ln\u00ED syst\u00E9m steriln\u00EDho hybridu S. townsendii a z n\u011Bj derivovan\u00E9 S. anglica, jako jedin\u00FD na sv\u011Bt\u011B, umo\u017E\u0148uje poskytnout odpov\u011B\u010F na tuto ot\u00E1zku. 2. Epigenetick\u00E9 aspekty. V t\u00E9to \u010D\u00E1sti se budeme zab\u00FDvat problematikou epigenetick\u00E9ho uml\u010Dov\u00E1n\u00ED exprese rodi\u010Dovsk\u00FDch gen\u016F rRNA v r\u016Fzn\u00FDch populac\u00EDch S. anglica a S. townsendii. Budeme \u0159e\u0161it ot\u00E1zky, zda fenom\u00E9n nukleol\u00E1rn\u00ED dominance (t\u00E9\u017E transkrip\u010Dn\u00ED uml\u010Dov\u00E1n\u00ED genov\u00E9 exprese) je z\u00E1konit\u00FD nebo stochastick\u00FD, zda je ovliv\u0148ov\u00E1n popula\u010Dn\u00ED dynamikou, duplikac\u00ED chromosomov\u00FDch sad eventueln\u011B genov\u00FDmi p\u0159estavbami v pr\u016Fb\u011Bhu polyploidie a zda existuje korelace mezi uml\u010Dov\u00E1n\u00EDm a metylac\u00ED DNA." . "MEB021114" . . "0"^^ . "2013-06-28+02:00"^^ . "0"^^ . " evolution" . . "1"^^ . "This project is being realized in the framework of the MOBILITY Activity that aims primarily on establishing and strenghtening ties with foreign research institutions. The control of particular outputs is not implemented by the evalution committee, but the correctness of allocated finances and the adequacy of their use are checked."@en . "0"^^ . . " rDNA" . "0"^^ . "2011-01-01+01:00"^^ . "At the heart of the proposed work is the study of rRNA genes evolution and their expression activity in natural populations of S. anglica. The rationale is that this system allow us to investigate evolutionary processes operating over time scales that are not amenable to laboratory study. They each make use of hybridisation occurring in the wild as naturally occurring experimental manipulations. In the proposed project we ask the following questions: 1. The genetic aspects of rDNA evolution in Spartina anglica. Specifically we will address the question of genetic interaction and homogenisation of parental genes in different populations collected at various places of French and British coasts. We will ask whether the changes (if occurring) are triggered by interspecific hybridization or chromosome duplication. This hypothesis could be tested analyzing populations of sterile T. x townsendii hybrid and parental accessions S. maritima and S.arterniflora. 2. The epigenetic aspects of rDNA evolution in Spartina anglica. Specifically we will address the question of epigenetic silencing of parental rDNA units among populations of F1 hybrids and derived dodecaploids. We wonder whether the expression changes could be correlated with changes in cytosine methylation levels of homeologous units."@en . " allopolyploid" . . "Projekt je realizov\u00E1n v\u00A0r\u00E1mci Aktivity MOBILITY, jej\u00EDm\u017E hlavn\u00EDm c\u00EDlem je nav\u00E1z\u00E1n\u00ED a prohlubov\u00E1n\u00ED kontakt\u016F se zahrani\u010Dn\u00EDmi v\u00FDzkumn\u00FDmi institucemi. Neprob\u00EDh\u00E1 tedy kontrola d\u00EDl\u010D\u00EDch v\u00FDstup\u016F projektu prost\u0159ednictv\u00EDm hodnot\u00EDc\u00ED komise, av\u0161ak je kontrolov\u00E1na spr\u00E1vnost \u010Derp\u00E1n\u00ED p\u0159id\u011Blen\u00FDch financ\u00ED a p\u0159im\u011B\u0159enost jejich vyu\u017Eit\u00ED."@cs . "Struktura a exprese gen\u016F pro ribosom\u00E1ln\u00EDch RNA v p\u0159irozen\u00FDch populac\u00EDch alopolyploidn\u00ED Spartina anglica jako v\u00FDznamn\u00E9ho evropsk\u00E9ho rostlinn\u00E9ho invazivn\u00EDho druhu." .