"ME10048" . . "The goal of the project is to understand the function of SUMO protein in the homologous recombination, which is the essential process for meiosis and error-free DNA repair of double-strand breaks."@en . . . . . . "V r\u00E1mci charakterizov\u00E1n\u00ED vlivu sumoylace na vlastnosti proteinu Lif1 jsme identifikovali lysin, na kter\u00E9m doch\u00E1z\u00ED ke konjugaci, a testovali vliv DNA a E3 lig\u00E1z na jeho sumoylaci. Anal\u00FDza pomoc\u00ED 2-hybridn\u00EDho syst\u00E9mu uk\u00E1zala vliv sumoylace na oligomerizaci Lif1 proteinu a na\u0161e v\u00FDsledky nazna\u010Duj\u00ED, \u017Ee sumoylace Lif1 sni\u017Euje \u00FA\u010Dinnost nehomologn\u00EDho spojov\u00E1n\u00ED konc\u016F a p\u0159edstavuje nov\u00FD regula\u010Dn\u00ED element."@cs . . . . . "http://www.isvav.cz/projectDetail.do?rowId=ME10048"^^ . . "C\u00EDlem projektu je pochopen\u00ED funkce SUMO proteinu v procesu homologn\u00ED rekombinace, kter\u00FD je nezbytnou sou\u010D\u00E1st\u00ED meiotick\u00E9ho d\u011Blen\u00ED bun\u011Bk umo\u017E\u0148uj\u00EDc\u00EDho pohlavn\u00ED rozmno\u017Eov\u00E1n\u00ED a z\u00E1rove\u0148 je jedn\u00EDm z kl\u00ED\u010Dov\u00FDch mechanism\u016F zaji\u0161\u0165uj\u00EDc\u00EDch opravu po\u0161kozen\u00E9 DNA." . . "Vliv post-transla\u010Dn\u00EDch modifikac\u00ED na DNA opravu a rekombinaci." . " recombination" . "During characterization of the role of Lif1 sumoylation we have identified main conjugation lysine and tested the effect of DNA and E3 ligases on its sumoylation. Using 2-hybrid system we have shown the effect of sumoylation on the oligomerization of Lif1 protein and our data indicate that sumoylation down-regulates the efficiency of end joining and represents a new regulatory element."@en . "SUMOylation" . . "2013-06-28+02:00"^^ . "9"^^ . "9"^^ . . "2010-05-01+02:00"^^ . . "0"^^ . "The role of post-translational modification in DNA repair and recombination: Meiosis, SUMOylation and Zip3 protein"@en . " DNA repair" . "1"^^ . "SUMOylation; DNA repair; recombination; meiosis"@en . . "2012-02-16+01:00"^^ . "2012-12-31+01:00"^^ . . . "0"^^ . .