. "Poprv\u00E9 na sv\u011Bt\u011B jsme 3D BiplaneFPALM mikroskopi\u00ED s rozli\u0161en\u00EDm 25 nm zobrazili s\u00ED\u0165 mitochondri\u00E1ln\u00EDho retikula, a to is spole\u010Dn\u011B s objekty do nich\u017E se sdru\u017Euje mitochondri\u00E1ln\u00ED DNA se jej\u00EDmi strukturn\u00EDmi protein \u2013 s nukleoidy mtDNA."@cs . . "0"^^ . . "Time resolved studies of living cells by conventional confocal microscopy will be followed by \u201Csnapshots\u201D of fixed cells by 3D high resolution (100 nm) 4Pi microscopy and by superresolution technique invented by the cooperating partner - by BiplaneFPALM. Thus 3D imaging of mitochondrial network morphology in cells and 3D imaging of superposition of mitochondrial nucleoids (clusters of mtDNA) will be achieved. Fluorescent protein (FP)-conjugates of ATAD3 and TFAM nucleoid proteins will be used for vizualization, such PA-CFP2-ATAD3 and Eos-TFAM. Membrane countours will be attempted by FP-conjugated membrane marker proteins. Mitochondrial nucleoid distribution will be studied with the regards fusion-fission cycles and cell division."@en . . "1"^^ . . "high resolution microscopy mitochondrial DNA nucleoids (clusters) of mitochondrial DNA 3D imaging of mitochondrial network morphology in cells 3D imaging of mitochondrial nucleoids 4Pi microscopy BiplaneFPALM microscopy"@en . . . "Vyu\u017Eit\u00ED 3D mikroskopick\u00FDch technik s vysok\u00FDm rozli\u0161en\u00EDm pro vizualizaci substruktury mitochondri\u00ED" . . . "5"^^ . "2013-06-28+02:00"^^ . . . "5"^^ . . . "2009-04-01+02:00"^^ . "V\u00DDVOJ METODIK MIKROSKOPIE S VYSOK\u00DDM ROZLI\u0160EN\u00CDM JAKO JE 4Pi MIKROSKOPIE A NOV\u00C9 SUPERREZOLUJ\u00CDC\u00CD MIKROSKOPICK\u00C9 TECHNOLOGIE BiplaneFPALM, VE SPOJEN\u00CD SE STUDIEMI NEPROB\u00C1DAN\u00C9HO MIKROSV\u011ATA NUKLEOID\u016E MITOCHONDRI\u00C1LN\u00CD DNA A VZHLEDEM K HETEROGENEIT\u011A MUTOVAN\u00C9 mtDNA." . . "2012-02-23+01:00"^^ . . "Imaging of mitochondrial substructure by 3D high resolution microscopy"@en . . "With a word priority we have for the first time obtained 3D images with 25 nm resolution of the mitochondrial network together with the nucleoids of mitochondrial DNA."@en . "ME09029" . "2012-12-31+01:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=ME09029"^^ . . . "0"^^ . .