. "Struktura a funkce interf\u00E1zn\u00EDch chromosom\u016F" . "1"^^ . . . "0"^^ . . . . . . . "0"^^ . . "Structure and function of interphase chromosomes"@en . . . . "Bude studov\u00E1na struktura chromosom\u016F u interf\u00E1zn\u00EDch 3D-fixovan\u00FDch bun\u011Bk u\u017Eit\u00EDm FISH techniky a cytometrie s vysok\u00FDm rozli\u0161en\u00EDm pro n\u011Bkolik zdrav\u00FDch a n\u00E1dorov\u00FDch bun\u011B\u010Dn\u00FDch l\u00EDni\u00ED s odli\u0161n\u00FDm stupn\u011Bm progrese. Sou\u010Dasn\u011B budou stanoveny transkrip\u010Dn\u00ED mapy u\u017Eit\u00EDm, technologie DNA-mikro\u010Dip\u016F. Srovn\u00E1n\u00ED t\u011Bchto map by n\u00E1m m\u011Blo umo\u017Enit nalezen\u00ED gen\u016F, kter\u00E9 jsou odpov\u011Bdn\u00E9 za kancerogenezi. Zm\u011Bny struktury, metylace a acetylace chromosom\u016F vzhledem ke zdrav\u00E9 tk\u00E1ni budou srovn\u00E1v\u00E1ny se zm\u011Bnami exprese. Bude u\u010Din\u011Bn z\u00E1v\u011Br ovz, ztahu mezi strukturou chromosom\u016F, acetylac\u00ED histon\u016F a expres\u00ED gen\u016F b\u011Bhem diferenciace a kancerogeneze.," . . "sledov\u00E1n\u00ED zm\u011Bn v expresi mezi kolorekt\u00E1ln\u00EDm karcinomem a norm\u00E1ln\u00EDm epeitelem"@cs . . . "The structure of chromosomes will be investigated in interphase 3D-fixed nuclei by means of FISH and high-resolution cytometry for several normal and tumor cell populations in different stages of progression. In parallel, using DNA-microarray technology,, , the transcriptome maps will be determined. Comparison of these maps should allow us to find out combinations of genes related to cancerogenesis. Changes of the structure, methylation and acetylation relatively to the normal tissue will be compared with, the changes of gene expression. Conclusion will be made on the relationship between the structure of chromosomes, histone acetylation and expression of genes during differentiation and cancerogenesis.,"@en . "chromosome structure" . "2008-06-02+02:00"^^ . . " DNA-microarrays" . "chromosome structure; FISH; high-resolution cytometry; DNA-microarrays; cancer"@en . . "16"^^ . . " FISH" . "16"^^ . "ME 565" . "observation of changes in expresion betweem colorectal carcinoma nd normal epitel"@en . " high-resolution cytometry" . "http://www.isvav.cz/projectDetail.do?rowId=ME 565"^^ . . .