"Tolerance po\u0161kozen\u00ED DNA u rostlin" . "alkylation DNA damage" . . "ME 535" . "Anal\u00FDza sekvence cel\u00E9ho genomu Arabidopsis uk\u00E1zala p\u0159\u00EDtomnost n\u011Bkolika homolog\u016F DNA polymeras, kter\u00E9 jsou b\u011Bhem replikace schopn\u00E9 tolerovat n\u011Bkter\u00E1 po\u0161kozen\u00ED DNA v bakteri\u00EDch a u savc\u016F. Toto zji\u0161t\u011Bn\u00ED podporuje na\u0161e d\u0159\u00EDv\u011Bj\u0161\u00ED pozorov\u00E1n\u00ED, \u017Ee alkyla\u010Dn\u00ED po\u0161ko, zen\u00ED DNA u rostlin je pravd\u011Bpodobn\u011B sp\u00ED\u0161e tolerov\u00E1no ne\u017E reparov\u00E1no. Projekt je zam\u011B\u0159en na studium funkce kl\u00ED\u010Dov\u00E9ho enzymu polymerasy theta v diferencovan\u00FDch a rostouc\u00EDch tk\u00E1n\u00EDch Arabidopsis pomoc\u00ED srovn\u00E1n\u00EDm divok\u00E9ho typu a inser\u010Dn\u00EDho Pol- mutanta, kter\u00FD, bude isolov\u00E1n.," . . "Sequence analysis of Arabidopsis genome revealed several homologues of DNA polymerases identified in bacteria and mammals that tolerate several kinds of DNA damage during replication. This finding support previous observation that in plants alkylation da, mage is rather tolerated than repaired. We propose to study activity of a key enzyme - Pol theta in regard to presence of alkylation damage in DNA of differentiated or dividing Arabidopsis tissue. For this purpose Pol- insertion mutant will be isolated., ,"@en . "0"^^ . "2"^^ . . . "2"^^ . . "1"^^ . "http://www.isvav.cz/projectDetail.do?rowId=ME 535"^^ . "DNA damage tolerance by plants"@en . "2008-06-03+02:00"^^ . "insertion mutants" . . . "DNA polymerases" . . . "0"^^ . . . . "DNA polymerases;alkylation DNA damage;insertion mutants;Arabidopsis"@en . . "Klonov\u00E1n gen DNA polymerasy kappa Arabidopsis thaliana a p\u0159ipraveny vektroy pro jeho produkci a kompletaci v bakteri\u00EDch a sav\u010D\u00EDch bu\u0148k\u00E1ch.Z\u00EDsk\u00E1ny t\u0159i T-DNA iner\u010Dn\u00ED linie Arabidopsis pro studium mechanismu reparace a metageneze u rostlin."@cs . . . . . .