. "2014-02-03+01:00"^^ . "Projekt je zam\u011B\u0159en na vyu\u017Eit\u00ED modern\u00EDch, vysoce selektivn\u00EDch a extr\u00E9mn\u011B citliv\u00FDch analytick\u00FDch metod pro objas\u0148ov\u00E1n\u00ED role hormon\u016F p\u0159i regulaci v\u00FDvojov\u00FDch proces\u016F v rostlin\u00E1ch. Za t\u00EDmto \u00FA\u010Delem bude pou\u017Eito c\u00EDlen\u00E9 metabolomick\u00E9 anal\u00FDzy v\u0161ech hlavn\u00EDch skupin fytohormon\u016F za sou\u010Dasn\u00E9ho stanoven\u00ED co nejv\u011Bt\u0161\u00EDho po\u010Dtu jednotliv\u00FDch metabolit\u016F. Tento p\u0159\u00EDstup vy\u017Eaduje v\u00FDvoj nov\u00FDch postup\u016F izolace (extrakce i purifikace) a optimalizaci metod pracuj\u00EDc\u00EDch se spojen\u00FDmi technikami ultra \u00FA\u010Dinn\u00E9 kapalinov\u00E9 chromatografie a tandemov\u00E9 hmotnostn\u00ED spektrometrie. Pro spr\u00E1vn\u00E9 a p\u0159esn\u00E9 soub\u011B\u017En\u00E9 stanoven\u00ED auxin\u016F, cytokinin\u016F, giberelin\u016F, kyseliny abscisov\u00E9 a jejich metabolit\u016F, brassinosteroid\u016F a jasmon\u00E1t\u016F bude nutn\u00E1 i p\u0159\u00EDprava n\u011Bkter\u00FDch nov\u00FDch stabiln\u011B izotopicky zna\u010Den\u00FDch standard\u016F. Vyvinut\u00E9 metodiky budou validov\u00E1ny p\u0159i stanoven\u00ED celkov\u00E9ho hormon\u00E1ln\u00EDho metabolomu v r\u016Fzn\u00FDch \u010D\u00E1stech rostlin (ko\u0159en, nadzemn\u00ED \u010D\u00E1st, kv\u011Bty) u rozli\u010Dn\u00FDch ekotyp\u016F a nadprodukuj\u00EDc\u00EDch mutantn\u00EDch lini\u00EDch Arabidopsis thaliana. Porovn\u00E1n\u00EDm d\u00EDl\u010D\u00EDch a vz\u00E1jemn\u00FDch posun\u016F v hormon\u00E1ln\u00EDch hladin\u00E1ch z\u00EDsk\u00E1me odpov\u011Bdi na ot\u00E1zky kladen\u00E9 v oblastech v\u00FDvojov\u00E9 biologie, biochemie a fyziologie rostlin." . "14"^^ . . "14"^^ . "http://www.isvav.cz/projectDetail.do?rowId=LK21306"^^ . . . "phytormone profiling" . "0"^^ . "phytormone profiling, plant growth regulators, mass sectrometry, ultra performance liquid chromatography, immunoaffinity extraction"@en . "2015-12-31+01:00"^^ . "The main aim of the project is application of modern, high selective and extremely sensitive analytical tools for study of the hormone function in plant growth and development. We will use the phytohormone profiling approaches (targeted metabolomics) for screening of all phytohormone groups in minute amount of fresh plant material. Development of new isolation methods (extraction and purification) from one sample will be combining with optimization of analytical methods \u2013 ultra high performance chromatography and tandem mass spectrometry. Preparation of stable isotope-labelled internal standards is also part of the project to simultaneously profile the majority of known plant growth regulators (auxins, cytokinins, gibberellins, abscisic acid, brassinosteroids and jasmonates). We will validate the method by profiling the phytohormone metabolome in different tissues (roots, shoots and flowers) from various Arabidopsis thaliana ecotypes and over-producing mutant lines. Use of our procedure and comparison of the hormonal levels together can be beneficial for plant biologists, biochemists and physiologists."@en . . " ultra performance liquid chromatography" . "2015-02-16+01:00"^^ . . "1"^^ . "Targeted Metabolite Profiling of Plant Growth Regulators"@en . . . . . . "C\u00EDlen\u00E9 metabolick\u00E9 profilov\u00E1n\u00ED rostlinn\u00FDch r\u016Fstov\u00FDch regul\u00E1tor\u016F" . "LK21306" . "0"^^ . . . "2013-02-01+01:00"^^ . " mass sectrometry" . . " plant growth regulators" . . . .