"Chemick\u00E1 a elektrochemick\u00E1 anal\u00FDza purinov\u00FDch deriv\u00E1t\u016F a jejich komplex\u016F s m\u011Bd\u00ED" . "0"^^ . " Cu(I)-purine complexes" . . . "Projekt je zam\u011B\u0159en na v\u00FDvoj jednoduch\u00FDch a citliv\u00FDch za\u0159\u00EDzen\u00ED pro diagnostiku purinov\u00FDch deriv\u00E1t\u016F. Jejich stanoven\u00ED se v posledn\u00ED dob\u011B stalo d\u016Fle\u017Eitou sou\u010D\u00E1st\u00ED klinick\u00E9 biochemie a biomedic\u00EDny. I kdy\u017E zna\u010Dn\u00E9 \u00FAsil\u00ED bylo v\u011Bnov\u00E1no detekci b\u011B\u017En\u00FDch purin\u016F pomoc\u00ED vysoce \u00FA\u010Dinn\u00E9 kapalinov\u00E9 chromatografie (HPLC) a kapil\u00E1rn\u00ED elektrofor\u00E9zy (CE), jsou tyto analytick\u00E9 metody ve srovn\u00E1n\u00ED s metodou elektrochemickou slo\u017Eit\u00E9 a pom\u011Brn\u011B drah\u00E9. Elektrochemick\u00FD senzor je jednodu\u0161\u0161\u00ED, n\u00E1kladov\u011B efektivn\u00ED a \u010Dasov\u011B \u00FAsporn\u00FD, vhodn\u00FD i pro detekci purin\u016F ve sm\u011Bsi vzork\u016F. V\u00FDhodou senzoru je tak\u00E9 mo\u017Enost automatizace, miniaturizace a paraleln\u00ED anal\u00FDzy v\u00EDce vzork\u016F (sn\u00EDma\u010D pole). Nicm\u00E9n\u011B, redoxn\u00ED vlastnosti mnoha purinov\u00FDch deriv\u00E1t\u016F nejsou zcela zn\u00E1my a jejich studium z hlediska analytick\u00E9 pou\u017Eitelnosti je \u017E\u00E1douc\u00ED. Jako elektrodov\u00FDch materi\u00E1l\u016F za t\u00EDmto \u00FA\u010Delem p\u0159edpokl\u00E1d\u00E1me vyu\u017Eit\u00ED uhl\u00EDkov\u00FDch elektrod v r\u016Fzn\u00FDch modifikac\u00EDch. V\u00FDhodou uhl\u00EDkov\u00FDch materi\u00E1l\u016F je jejich dobr\u00E1 vodivost, biokompatibilita, chemick\u00E1 inertnost a relativn\u011B vysok\u00E1 specifick\u00E1 plocha povrchu. Hlavn\u00ED c\u00EDl projektu je realizace my\u0161lenky vytvo\u0159it na povrchu elektrody (in situ) m\u00E1lo rozpustn\u00E9, Cu(I)-purinov\u00E9 komplexy, kter\u00E9 se dob\u0159e na elektrodov\u00E9m povrchu adsorbuj\u00ED a t\u00EDm i akumuluj\u00ED. Pro zkoncentrov\u00E1n\u00ED komplexu vyu\u017Eijeme adsorptivn\u00ED rozpou\u0161t\u011Bc\u00ED voltametrii (AdSV) nebo adsorptivn\u00ED p\u0159enosovou rozpou\u0161t\u011Bc\u00ED voltametrii (AdTSV) a v kombinaci s elimina\u010Dn\u00ED voltametri\u00ED s line\u00E1rn\u00ED polarizac\u00ED (EVLS)dos\u00E1hneme velmi n\u00EDzk\u00FDch detek\u010Dn\u00EDch limit\u016F (nanomol\u00E1rn\u00ED a subnanomol\u00E1rn\u00ED koncentrace). Takto navr\u017Een\u00E9 senzory pro purinov\u00E9 deriv\u00E1ty mohou naj\u00EDt \u0161irok\u00E9 uplatn\u011Bn\u00ED v mnoha v\u00FDzkumn\u00FDch oblastech, jako je biofyzika, biochemie, medic\u00EDna, klinick\u00E1 diagnostika, l\u00E9k\u00E1rensk\u00E1 praxe." . . "Purine derivatives, graphite electrodes, Cu(I)-purine complexes, electrochemical sensors"@en . . "2014-02-28+01:00"^^ . "2013-03-15+01:00"^^ . . . . . "2015-02-16+01:00"^^ . "LH13053" . "Chemical and electrochemical analysis of purine derivatives and their copper complexes"@en . . . . "2015-12-31+01:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=LH13053"^^ . . . . . "11"^^ . "1"^^ . "11"^^ . "The project is focused on the development of simple and sensitive diagnostic devices of purine derivatives. The determination of purine derivatives has recently become an important part of clinical biochemistry and biomedicine. Although considerable effort has been devoted to the detection of normal purines using high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), the following analytical methods are compared with the electrochemical method is relatively complex and expensive. The electrochemical sensor is a simple, cost-effective and time-saving and well suited for the detection of purines in the mixture samples. The advantage of the sensor is also the possibility of automation, miniaturization and parallel analysis of multiple samples (sensor array). However, the redox properties of many purine derivatives are not fully known and studied in terms of analytical application is desirable. As electrode materials for this purpose, we assume the use of graphite electrodes in various modifications. The advantage of carbon materials is their good conductivity, biocompatibility, chemical inertness and relatively high specific surface area. The main goal of the project is the realization of the idea of \u200B\u200Bcreating the electrode surface (in situ) slightly soluble Cu (I)-purine complexes, which are easily adsorbed on the electrode surface and thus accumulated. For the increase of the complex concentration will be used adsorptive stripping voltammetry (AdSV) or adsorptive transfer stripping voltammetry (AdTSV) in combination with elimination voltammetry with linear polarization (EVLS). We achieve very low detection limits (nanomolar and subnanomolar concentrations). Thus, the proposed sensors for purine derivatives may find wide use in many research areas such as biophysics, biochemistry, medicine, clinical diagnostics, pharmaceutical practice."@en . . " graphite electrodes" . "0"^^ . "Purine derivatives" . .