. . "0"^^ . " microsatellite markers" . . "Musaceae; gene bank; genetic diversity; ploidy level; genome size; microsatellite markers; high-throughput capillary electrophoresis; sequencing; ITS region of 45S rDNA"@en . " gene bank" . "0"^^ . "2"^^ . "2"^^ . "1"^^ . " sequencing" . . . . . "Musaceae" . " genome size" . . . "http://www.isvav.cz/projectDetail.do?rowId=LG12021"^^ . " high-throughput capillary electrophoresis" . "C\u00EDlem projektu je prohloubit a roz\u0161\u00ED\u0159it spolupr\u00E1ci s Bioversity International a charakterizovat genetickou diversitu z\u00E1stupc\u016F \u010Deledi Musaceae uchov\u00E1van\u00FDch ve sv\u011Btov\u00E9 genov\u00E9 bance (asi 500 polo\u017Eek) a to v\u010Detn\u011B nov\u00FDch polo\u017Eek, kter\u00E9 budou v dob\u011B \u0159e\u0161en\u00ED projektu do banky za\u0159azov\u00E1ny (asi 150 polo\u017Eek). Anal\u00FDzy budou prov\u00E1d\u011Bny s vyu\u017Eit\u00EDm genotypovac\u00ED platformy v laborato\u0159i \u0159e\u0161itele. D\u00EDky komplexn\u00EDmu p\u0159\u00EDstupu, kter\u00FD krom\u011B genotypov\u00E1n\u00ED pomoc\u00ED 19 mikrosatelitov\u00FDch marker\u016F vyu\u017E\u00EDv\u00E1 informace o ploidii budou identifikov\u00E1ny polo\u017Eky obsahuj\u00EDc\u00ED v\u00EDce genotyp\u016F, mixoploidn\u00ED polo\u017Eky, nespr\u00E1vn\u011B popsan\u00E9 polo\u017Eky a duplikovan\u00E9 polo\u017Eky. U polo\u017Eek, u kter\u00FDch genotypov\u00E1n\u00ED pomoc\u00ED mikrosatelitov\u00FDch marker\u016F neposkytne jednozna\u010Dn\u00E9 v\u00FDsledky, budou analyzov\u00E1ny nukleotidov\u00E9 sekvence kr\u00E1tk\u00E9ho mezern\u00EDku 45S rRNA gen\u016F (ITS sekvence). Z\u00EDskan\u00E1 data budou pou\u017Eita pro fylogenetickou anal\u00FDzu s c\u00EDlem objasnit p\u016Fvod p\u011Bstovan\u00FDch forem ban\u00E1novn\u00EDku." . . . . . "LG12021" . . . "This project will contribute to the conservation and use of genetic diversity of bananas and plantains. The investigations will include all accessions held in the global gene bank as well as the accessions which will be newly introduced to the bank. The experiments will be performed using the recently established genotyping platform, and will include flow cytometric estimation of ploidy and genome size, and analysis using 19 microsatellite (SSR) markers. The accessions, which will not give clear results after SSR genotyping, will be used for sequence analysis of ITS locus. This complex approach will result in detailed description of genetic diversity and facilitate identification of mixed and mixoploid accessions in the gene bank. The results will shed light on the evolution and genomic constitution of edible banana clones and characterize genetic diversity and phylogenetic relationships within the family Musaceae."@en . "Collaboration with Bioversity International on global analysis and conservation of genetic diversity of bananas"@en . "2013-02-18+01:00"^^ . . "2014-10-06+02:00"^^ . "Spolupr\u00E1ce s Bioversity International na glob\u00E1ln\u00ED anal\u00FDze a uchov\u00E1v\u00E1n\u00ED genetick\u00E9 diverzity ban\u00E1novn\u00EDku" . " ploidy level" . . "2012-03-01+01:00"^^ . . "2014-12-31+01:00"^^ . " genetic diversity" .