. . "Cherry virome deep sequencing analysis and development of virus specific PCR detection tools."@en . . . . "LD14004" . . "2016-04-30+02:00"^^ . " phytoplasma" . . " virus" . . . "next generation sequencing; virus; phytoplasma; cherry; sour cherry"@en . . . . . "2015-04-29+02:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=LD14004"^^ . . . "Anal\u00FDza viromu t\u0159e\u0161n\u00ED a vi\u0161n\u00ED sekvenov\u00E1n\u00EDm nov\u00E9 generace a v\u00FDvoj n\u00E1stroj\u016F pro specifickou detekci vir\u016F PCR." . "http://www.umbr.cas.cz"^^ . "2014-05-19+02:00"^^ . "next generation sequencing" . . . "Using parallel sequencing (next generation sequencing), RNA virome of sweet and sour cherry will be analysed. At the same time, phytoplasmas infections resembling symptoms of virus infection will be detected by PCR. Newly found viruses will be molecularly characterized and classified, and con-served or variable parts of genomes will be reported. Genetic variability of identified phytoplasmas will be analysed. For all pathogens discovered, specific primers will be developed allowing detection of these from total RNA. The cases of virus and phytoplasma mixed infections will be recorded."@en . "0"^^ . " cherry" . "0"^^ . "1"^^ . "0"^^ . "0"^^ . "2014-04-01+02:00"^^ . "Pomoc\u00ED paraleln\u00EDho sekvenov\u00E1n\u00ED (next generation sequencing) bude analyzov\u00E1n RNA virom t\u0159e\u0161n\u00ED a vi\u0161n\u00ED. Z\u00E1rove\u0148 bude pomoc\u00ED PCR zji\u0161t\u011Bn v\u00FDskyt fytoplazem, kter\u00E9 mohou vyvol\u00E1vat p\u0159\u00EDznaky podobn\u00E9 virov\u00FDm infekc\u00EDm. Nov\u011B nalezen\u00E9 viry \u010Di jejich kmeny budou molekul\u00E1rn\u011B pops\u00E1ny a za\u0159azeny, na jejich genomu budou vyhled\u00E1v\u00E1ny konzervovan\u00E9 a variabiln\u00ED \u00FAseky. U identifikovan\u00FDch fytoplazem bude charakterizov\u00E1na genetick\u00E1 variabilita. Na z\u00E1klad\u011B v\u00FDsledk\u016F budou pro \u00FA\u010Dely rutinn\u00ED detekce vyvinuty specifick\u00E9 primery detekuj\u00EDc\u00ED v\u0161echny zji\u0161t\u011Bn\u00E9 patogeny z celkov\u00E9 RNA. Budou pops\u00E1ny p\u0159\u00EDpadn\u00E9 sm\u011Bsn\u00E9 infekce vir\u016F a fytoplazem." .