"Vyu\u017Eit\u00ED p\u0159\u00EDstup\u016F metagenomiky a metatraskriptomiky k charakterizaci mikrobi\u00E1ln\u00ED diverzity \u010Dlov\u011Bkem ovlivn\u011Bn\u00FDch p\u016Fd" . "0"^^ . "2014-02-27+01:00"^^ . " human impacted soil" . "Application of metagenomics and metatranscriptomics for evaluation of microbial diversity of human impacted soils"@en . "0"^^ . . "C\u00EDlem projektu je zhodnotit diverzitu p\u016Fdn\u00EDho mikrobi\u00E1ln\u00EDho spole\u010Denstva v rozli\u010Dn\u00FDch ekosyst\u00E9mech ovlivn\u011Bn\u00FDch lidskou \u010Dinnost\u00ED (intenzivn\u00ED zem\u011Bd\u011Blstv\u00ED souvisej\u00EDc\u00ED s organick\u00FDm hnojen\u00EDm a vysu\u0161ov\u00E1n\u00EDm, odled\u0148ov\u00E1n\u00ED souvisej\u00EDc\u00ED s oteplov\u00E1n\u00EDm klimatu) pomoc\u00ED 454 pyroskevenov\u00E1n\u00ED. Budou vyvinuty a standardizov\u00E1ny pracovn\u00ED postupy pro amplikonov\u00E9 i shotgunov\u00E9 sekvenov\u00E1n\u00ED, tak jako i bioinformatick\u00E9 n\u00E1stroje zpracov\u00E1n\u00ED a anotace sekvenc\u00ED, ve spolupr\u00E1ci se zahrani\u010Dn\u00EDmi partnery. Na\u0161imi hlavn\u00EDmi c\u00EDli budou a) vyvinout metodu extrakce RNA pro p\u016Fdy a hlubok\u00E9 vrstvy p\u016Fd a tak\u00E9 pro hnojen\u00E9 p\u016Fdy a prov\u00E1d\u011Bt metatraskriptomov\u00E9 studie aktivn\u00EDch mikrobi\u00E1ln\u00EDch spole\u010Denstev in situ nebo v p\u016Fdn\u00EDch mikrokosmech, d\u00E1le b) vyvinout n\u00E1stroje pro sekvenov\u00E1n\u00ED a anotaci plazmid\u016F z\u00EDskan\u00FDch z p\u016Fd. Na\u0161e \u00FAsil\u00ED bude v\u011Bnov\u00E1no tak\u00E9 do podpory a povzbuzen\u00ED k vyu\u017E\u00EDv\u00E1n\u00ED metagenomiky a metatraskriptomiky v\u00FDzkumn\u00FDmi pracovn\u00EDky a studenty v na\u0161ich laborato\u0159\u00EDch a vyv\u00EDjet udr\u017Eitelnou spolupr\u00E1ci v t\u00E9to oblasti v\u00FDzkumu." . . "http://www.isvav.cz/projectDetail.do?rowId=LD13046"^^ . . "LD13046" . . . "The aim of the project is to evaluate diversity of soil microbial community in different ecosystems influenced by human activity (intesive agriculture associated with organic manuring and drying, bare soils after receding of glaciers) with help pf 454 pyrosequencing. The protocols and workflow will be developed, standartized and adopted for amplicon and shot-gun sequencing projects as well as bioinformatics tools for sequence data analysis and annotation in frame of cooperation with abroad partners. Our main tasks will be: a) develop RNA extraction method from soil, deep subsurface horizons of soil, manured soils and perform metatrascriptome studies of active microbial communities in situ or in soil microcosms, and b) to develop tools and methods for soil derived plasmid DNA sequencing and annotation. Our effort will be invested into the support of students and the practical usage of metagenomic and metatranscriptomic tools by researcher and students in our laboratories as well as development of sustainable cooperation in this topic."@en . . "2015-04-29+02:00"^^ . . . . . . . "2015-10-31+01:00"^^ . "microbial communities; human impacted soil; diversity; next gen sequencing"@en . . . . "1"^^ . . "0"^^ . . " diversity" . "microbial communities" . "0"^^ . "2013-03-21+01:00"^^ .