. . . "2015-06-30+02:00"^^ . "ecology; next-generation-sequencing; biodiversity; metagenomics; metatranscriptomics; soil ecology"@en . "2014-03-03+01:00"^^ . . . . . . "The use of metagenomics and metatranscriptomics for identification of active microorganisms and microbial processes"@en . . " next-generation-sequencing" . . "Projekt je zam\u011B\u0159en na identifikaci aktivn\u00EDch \u010Dlen\u016F mikrobi\u00E1ln\u00EDch spole\u010Denstev p\u016Fdy, na z\u00E1klad\u011B anal\u00FDzy rRNA a mRNA transkript\u016F metodami next-generation-sequencing. Budou vyvinuty protokoly pro reproducibiln\u00ED pr\u00E1ci s environment\u00E1ln\u00EDmi metatranskriptomy, zejm\u00E9na pro eukaryotn\u00ED mikroorganismy. \u00DAdaje o taxonomick\u00E9m slo\u017Een\u00ED spole\u010Denstev a o abundanci jednotliv\u00FDch taxon\u016F budou kombinov\u00E1ny s anotac\u00ED sekvenc\u00ED z transkriptom\u016F, aby bylo mo\u017En\u00E9 identifikovat pod\u00EDl jednotliv\u00FDch skupin mikroorganism\u016F na environment\u00E1ln\u00EDch procesech. Kombinace genomov\u00FDch sekvenc\u00ED s transkriptomikou za definovan\u00FDch podm\u00EDnek a specifick\u00E1 identifikace druhov\u011B-specifick\u00FDch sekvenc\u00ED v metatranskriptomech p\u0159isp\u011Bje k objasn\u011Bn\u00ED autekologie vybran\u00FDch mikroorganism\u016F. Projekt bude rovn\u011B\u017E zahrnovat systematick\u00FD pr\u016Fzkum vybran\u00FDch p\u016Fd z hlediska biogeografie mikroorganism\u016F na celoevropsk\u00E9 \u00FArovni." . "The project aims to identify the active microbial community represented by the RNA pool and transcriptionally active in the environment using next-generation-sequencing. The main challenge of the project is the development of protocols that will allow reproducible and reliable work with environmental metatranscriptomes, especially the eukaryotic ones. The information on the taxonomic composition of communities and the abundance of individual taxa will be combined with the assignment of transcriptome-derived sequences to analyse the contribution of individual groups of organisms to the processes in the environment. The combination of genome sequences of selected microbial taxa and model strains with transcriptomics under defined conditions and the specific search for the species-specific sequences will contribute to the understanding of the autecology of individual microbes. The project will also comprise systematic survey of important microbial habitats within a European framework."@en . "2015-04-29+02:00"^^ . . . " metagenomics" . "Kombinace p\u0159\u00EDstup\u016F metatranskriptomiky a metagenomiky pro identifikaci aktivn\u00EDch mikroorganism\u016F a jejich funkce v p\u016Fd\u011B" . . "3"^^ . "ecology" . "0"^^ . "3"^^ . . . " metatranscriptomics" . "1"^^ . . " biodiversity" . "http://www.isvav.cz/projectDetail.do?rowId=LD12048"^^ . . . "2012-03-01+01:00"^^ . "0"^^ . "LD12048" .