"2012-03-01+01:00"^^ . . . . "Dothistroma needle blight; brown spot needle bligth; spore trap; absolute quantification; Mycosphaerella pini; sexual stage; real time PCR"@en . . "LD12031" . "The project is focused on assembling of complex data about occurrence and biology of Dothistroma Neeedle Blight Dothistroma septosporum, D. pini and Brown Spot Needle Blight -Lecanosticta acicola in the Czech Republic and development (resp. validation) of methods developed by international cooperation in investigation of population structure, biology and treatment methods. The results will be consequently used for COST DIAROD. Partial goals are: 1. Monitoring of D. septosporum, D. pini and Lecanosticta acicola occurrence, arrangement of complex database in GIS, evaluation of occurrence by GIS, 2. Screening of presence D. septosporum vs D. pini. 3. Biology of Dothistroma spp. by use of spore trap, including validation of method based on absolute quantification of spores by real time PCR. 4. Biology and circumstances which are needed for initiation of sexual stage, importance of ascospores in pathogenesis, including characterisation of mycelium grow requirements in vitro."@en . . "C\u00EDlem projektu je z\u00EDskat, resp. zkompletovat ve\u0161ker\u00E1 dostupn\u00E1 data, kter\u00E1 se t\u00FDkaj\u00ED v\u00FDskytu a biologie p\u016Fvodc\u016F \u010Derven\u00E9 a hn\u011Bd\u00E9 sypavky Dothistroma septosporum, D. pini a Lecanosticta acicola v \u010Cesk\u00E9 republice a rozvoj, resp. validace metodik, kter\u00E9 jsou vyv\u00EDjeny v r\u00E1mci mezin\u00E1rodn\u00EDho v\u00FDzkumu popula\u010Dn\u00ED struktury, biologie a n\u00E1vrhu ochrann\u00FDch opat\u0159en\u00ED. V\u00FDsledky budou pak sou\u010D\u00E1st\u00ED \u0159e\u0161en\u00ED projektu COST DIAROD. D\u00EDl\u010D\u00EDmi c\u00EDly jsou 1. Monitoring vyskytu D.septosporum, D. pini a Lecanosticta acicola, zpracov\u00E1n\u00ED kompletn\u00ED datab\u00E1ze v GIS, vyhodnocen\u00ED v\u00FDskytu dle GIS, 2. Screening v\u00FDskytu D. septosporum vs D. pini. 3. Biologie Dothistroma spp. za pou\u017Eit\u00ED lapa\u010De spor, v\u010Detn\u011B validace metodiky kvantifikace mno\u017Estv\u00ED spor pomoc\u00ED real time PCR a za vyu\u017Eit\u00ED ploch, na kter\u00FDch byla provedena um\u011Bl\u00E1 infekce konidiemi, zhodnocen\u00ED citlivosti hostitel\u016F. 4. Biologie a podm\u00EDnky iniciace tvorby sexu\u00E1ln\u00EDho stadia, v\u00FDznam askospor v patogenezi, v\u010Detn\u011B charakteristiky r\u016Fstov\u00FDch podm\u00EDnek kmen\u016F in vitro." . "2015-04-29+02:00"^^ . "1"^^ . "Dothistroma needle blight" . " absolute quantification" . . "0"^^ . "0"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=LD12031"^^ . . "2014-03-03+01:00"^^ . . " brown spot needle bligth" . "Biology and distribution of Dothistroma spp. in the Czech republic"@en . " Mycosphaerella pini" . . . " sexual stage" . . "Biologie a roz\u0161\u00ED\u0159en\u00ED Dothistroma spp. v \u010Cesk\u00E9 republice" . . . " spore trap" . "5"^^ . "5"^^ . . . "2015-06-30+02:00"^^ . . .