" purification procedure" . "The process of photosynthesis cannot be understood without detailed knowledge of the structure of PSII and its single subunits. In order to designate molecular details of water oxidation catalyzed by the oxygen-evolving center (OEC) assembly, degradation, structure, function, and dynamic regulation of large protein-cofactor complex it is very important to crystallize and describe 3D structure of PS II from higher plants. No crystal structure of PS II from higher plants is available until now therefore our research is focused on structural studies of the membrane complex PSII. To develop a reproducible purification protocol controlling all chemical and physical parameters represents a complex task, however it is a crucial step toward reproducible crystallization experiments. Once this protocol is developed and optimized, we believe the pathway towards PS IICC crystallization and structure determination will be successful."@en . "LD11011" . . . . "1"^^ . . "1"^^ . "Projekt byl zam\u011B\u0159en na strukturn\u00ED studie fotosyst\u00E9mu II z\u00A0vy\u0161\u0161\u00EDch rostlin. Vzhledem k\u00A0tomu, \u017Ee krystalizovatelnost membr\u00E1nov\u00FDch komplex\u016F z\u00E1vis\u00ED na homogenit\u011B a stabilit\u011B vzork\u016F, byl v\u00FDzkum zam\u011B\u0159en na optimalizaci faktor\u016F ovliv\u0148uj\u00EDc\u00EDch tyto parametry a na kontrolu vzork\u016F biochemick\u00FDmi metodami. Byl navr\u017Een a optimalizov\u00E1n nov\u00FD purifika\u010Dn\u00ED protokol"@cs . "0"^^ . "Adventure and benefit from crystallization of higher plants membrane protein complex following its stuctural studies"@en . . "1"^^ . "0"^^ . . . . . "2011-03-01+01:00"^^ . "pigment-protein complex; photosystem II; Pisum sativum; purification procedure; crystallization methods; structural studies"@en . . . "V pr\u016Fb\u011Bhu \u0159e\u0161en\u00ED projektu bude v\u00FDzkum zam\u011B\u0159en na strukturn\u00ED studie membr\u00E1nov\u00E9ho komplexu PSII izolovan\u00E9ho z vy\u0161\u0161\u00EDch rostlin. Proto\u017Ee krystalizovatelnost membr\u00E1nov\u00FDch pigment-proteinov\u00FDch komplex\u016F je z\u00E1visl\u00E1 na jejich homogenit\u011B, velk\u00E1 pozornost bude v\u011Bnov\u00E1na faktor\u016Fm, kter\u00E9 by mohly zp\u016Fsobovat rozklad komplex\u016F, tak\u00FDmi jsou nap\u0159. p\u016Fsoben\u00ED vzduchu, absence manganu, vystaven\u00ED jin\u00E9mu ne\u017E zelen\u00E9mu sv\u011Btlu. Kl\u00ED\u010Dov\u00FD a kritick\u00FD krok k opakovan\u00E9 p\u0159\u00EDprav\u011B difraktuj\u00EDc\u00EDch krystal\u016F p\u0159edstavuje v\u00FDvoj reprodukovateln\u00E9ho purifika\u010Dn\u00EDho protokolu, kter\u00FD zohled\u0148uje a kontroluje v\u0161echny fyz-chem. podm\u00EDnky v komplexn\u00ED form\u011B. V p\u0159\u00EDpad\u011B, \u017Ee je tento protokol navr\u017Een a optimalizov\u00E1n, v\u011B\u0159\u00EDme, \u017Ee cesta k p\u0159\u00EDprav\u011B PSII krystal\u016F a vy\u0159e\u0161en\u00ED struktury pigment-proteinov\u00E9ho komplexu bude \u00FAsp\u011B\u0161n\u00E1." . . . . "pigment-protein complex" . "Adventure and benefit from crystallization of higher plants membrane protein complex following its stuctural studies / Riziko a p\u0159\u00EDnos z krystalizace membr\u00E1nov\u00E9ho proteinov\u00E9ho komplexu z vy\u0161\u0161\u00EDch rostlin prov\u00E1zej\u00EDc\u00ED jeho strukturn\u00ED studie" . "2013-12-31+01:00"^^ . "2014-06-30+02:00"^^ . " Pisum sativum" . " photosystem II" . . . . . "http://www.isvav.cz/projectDetail.do?rowId=LD11011"^^ . "The project was focused on structural studies of the higher plants photosystem II. As the crystallizability of membrane complexes depends on homogeneity and stability, the research was focused on optimization of factors influencing mentioned parameters and on biochemical samples control. New purification protocol was developed and optimized"@en . "2013-02-22+01:00"^^ . " crystallization methods" . .