. " quantiative evaluation" . . . " GFP-transformed tobacco cells" . . "We will test the applicability and efficiency of methods for estimating the length of ER tubules, their length density in cell, proportion of lamellar sheets in ER, mean size and size distribution of meshes in the ER net, characteristics of ER organization, anisotropy and texture. The quantitative evaluation of such characteristics together with ER three-dimensional reconstruction and visualization will provide a complex and objective information about its structure and thus will help in studies of ER changes, both in normally developing cells and cells suffering from various types of stress. The methods will be tested on GFP-transformed tobacco cells (line BY-2). The ER characteristics wil estimated by contemporary unbiased stereological methods and image analysis techniques applied to three-dimensional image data acquired by 2-photon and confocal microscopy."@en . " 3-D analysis" . . . "Image analysis methods for measuring the length density of endoplasmic reticulum (ER) tubules in tobacco cells were developed and implemented. Changes in ER structure were found during early phases of toxic influence of Al ions and latrunculin B."@en . . . "endoplasmic tericulum; 3-D analysis; 2-photopn microscopy; confocal microscopy; image analysis; quantiative evaluation; GFP-transformed tobacco cells; stereological methods"@en . "Byly vyvinuty a implementov\u00E1ny metody anal\u00FDzy obrazu pro m\u011B\u0159en\u00ED d\u00E9lkov\u00E9 hustoty tubul\u016F endoplazmatick\u00E9ho retikula (ER) tab\u00E1kov\u00FDch bun\u011Bk. Bylo zji\u0161t\u011Bno, \u017Ee v \u010Dasn\u00FDch f\u00E1z\u00EDch toxick\u00E9ho p\u016Fsoben\u00ED Al iont\u016F a Latrunkulinu B doch\u00E1z\u00ED ke zm\u011Bn\u00E1m struktury ER."@cs . . . . . . "Trojrozm\u011Brn\u00E1 anal\u00FDza struktury endoplazmatick\u00E9ho retikula na z\u00E1klad\u011B zpracov\u00E1n\u00ED obraz\u016F z 2-fotonov\u00E9ho a konfok\u00E1ln\u00EDho mikroskopu" . "2014-02-12+01:00"^^ . . "endoplasmic tericulum" . "KJB6011309" . "Three-dimensional analysis of endoplasmic reticulum structure based on processing of images captured by 2-photon and confocal microscopy"@en . "13"^^ . . . " image analysis" . "13"^^ . . . . " 2-photopn microscopy" . "1"^^ . "Budeme testovat pou\u017Eitelnost a \u00FA\u010Dinnost metod pro m\u011B\u0159en\u00ED d\u00E9lky tubul\u016F endoplazmatick\u00E9ho retikula (ER), jejich d\u00E9lkov\u00E9 hustoty v bu\u0148ce, pod\u00EDlu lamel\u00E1rn\u00EDch struktur v ER, pr\u016Fm\u011Brn\u00E9 velikosti a rozd\u011Blen\u00ED velikost\u00ED ok v ER s\u00EDti, charakteristik organizace, anizotropie a textury ER. Kvantitativn\u00ED vyhodnocen\u00ED t\u011Bchto charakteristik spolu s trojrozm\u011Brn\u00FDmi rekonstrukcemi ER poskytne komplexn\u00ED a objektivn\u00ED informace o struktu\u0159e ER, co\u017E p\u0159isp\u011Bje k podrobn\u011Bj\u0161\u00EDmu studiu jeho zm\u011Bn jak v norm\u00E1ln\u011B se vyv\u00EDjej\u00EDc\u00EDch bu\u0148k\u00E1ch, tak i v bu\u0148k\u00E1ch vystaven\u00FDch r\u016Fzn\u00FDm typ\u016Fm stresu. Tyto metody budou testov\u00E1ny na tab\u00E1kov\u00FDch bu\u0148k\u00E1ch linie BY-2 transformovan\u00FDch genem pro GFP. Charakteristiky ER budou odhadov\u00E1ny sou\u010Dasn\u00FDmi stereologick\u00FDmi metodami a technikami anal\u00FDzy obrazu aplikovan\u00FDmi na trojrozm\u011Brn\u00E1 obrazov\u00E1 data z\u00EDskan\u00E1 2-fotonovou a konfok\u00E1ln\u00ED mikroskopi\u00ED." . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=KJB6011309"^^ . . "2"^^ . . " confocal microscopy" .