. " chick embryo" . . "Ku\u0159ec\u00ED mesonefros funguje jako exkre\u010Dn\u00ED org\u00E1n v\u00EDce jak t\u0159etinu prenat\u00E1ln\u00EDho \u017Eivota. Nav\u00EDc obsahuje \u010Detn\u00E9 kmenov\u00E9 bu\u0148ky, v\u010Detn\u011B krvetvorn\u00FDch bun\u011Bk definitivn\u00ED s\u00E9rie. Na\u0161e v\u00FDsledky prok\u00E1zaly vysokou citlivost mesonefros k \u00FA\u010Dink\u016Fm DBE, souvisej\u00EDc\u00ED z\u0159ejm\u011B s po\u0161kozen\u00EDm c\u00E9vn\u00EDho syst\u00E9mu. K ov\u011B\u0159en\u00ED t\u00E9to hypot\u00E9zy pl\u00E1nujeme kvantifikaci plochy peritubul\u00E1rn\u00EDch c\u00E9v v kontrol\u00E1ch a ovlivn\u011Bn\u00FDch z\u00E1rodc\u00EDch pomoc\u00ED konfok\u00E1ln\u00ED mikroskopie a stereologick\u00E9 metody. N\u00E1sledn\u011B provedeme 3D rekonstrukci uspo\u0159\u00E1d\u00E1n\u00ED mesonefrick\u00FDch komponent. St\u00E1rnouc\u00ED lidsk\u00E9 erytrocyty vykazuj\u00ED intensivn\u00ED ztr\u00E1tu kyseliny sialov\u00E9 z membr\u00E1nov\u00FDch glykokonjug\u00E1t\u016F. Zd\u00E1 se, \u017Ee stejn\u00FD proces prov\u00E1z\u00ED i st\u00E1rnut\u00ED ku\u0159ec\u00EDch erytrocyt\u016F. Na\u0161e v\u00FDsledky s DBE nazna\u010Dily zm\u011Bny v krvetvorb\u011B ovlivn\u011Bn\u00FDch z\u00E1rodk\u016F. Abychom specifikovali tyto zm\u011Bny, budeme m\u011B\u0159it hladiny sializace membr\u00E1n v pr\u016Fb\u011Bhu diferenciace a st\u00E1rnut\u00ED erytroidn\u00EDch bun\u011Bk pomoc\u00ED lektinov\u00E9 histochemie, fluorescen\u010Dn\u00ED mikroskopie a po\u010D\u00EDta\u010Dov\u00E9 obrazov\u00E9 anal\u00FDzy." . "V\u00FDvoj c\u00E9vn\u00EDho syst\u00E9mu mesonefros a erytropoeza u ku\u0159ec\u00EDch z\u00E1rodk\u016F ovlivn\u011Bn\u00FDch 1,2-dibromoethanem t\u0159et\u00ED den inkubace" . "Development of mesonephric vascular system and erythropoiesis in the chick embryos exposed to 1,2-dibromoethane on incubation day 3"@en . . "2007-09-26+02:00"^^ . "mesonephric vascular stream development" . . . " 1" . . . . "Chick mesonephros functions as an excretory organ for more than third of prenatal life. In addition it contains multiple stem cells, including the hematopoietic cells of definitive series. Our results showed a high sensitivity of mesonephros to the action of DBE related probably to the damage to vascular system. To verify this assumption we shall quantify the area of peritubular vessels in the controls and treated embryos using confocal microscopy and stereological method. Then, 3D reconstruction of the spatial arrangement of mesonephric components will be performed. Senescent human erythrocytes exhibit an extensive desialylation of membrane glycoconujgates. The same process seems to follow aging of chick erythrocytes. Our data on DBE suggested the changes in erythropoiesis of exposed embryos. To specify these changes, we shall measure the levels of membrane sialylation during the blood cell differentiation using lectin histochemistry, fluorescence microscopy and image analysis system."@en . "V\u00FDrazn\u011Bj\u0161\u00ED posti\u017Een\u00ED diferenciace a r\u016Fstu ren\u00E1ln\u00EDch c\u00E9v oproti tubul\u00E1rn\u00EDmu apar\u00E1tu je p\u0159\u00ED\u010Dinou dysmorfogeneze mesonefros po p\u016Fsoben\u00ED DBE. Stupe\u0148 sializace bun\u011B\u010Dn\u00FDch membr\u00E1n se m\u011Bn\u00ED v pr\u016Fb\u011Bhu diferenciace erytrocyt\u016F v z\u00E1vislosti na bun\u011B\u010Dn\u00E9m cyklu."@cs . "2-dibromoethane" . " computerized image analysis" . . "0"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=KJB5039302"^^ . "More pronounced impairment of renal vessels when compared to tubules is responsible for DBE-induced dysmorphogenesis of mesonephros. The degree of cell membrane sialylation varies during differentiation of erythrocytes in dependence upon the cell cycle."@en . "2"^^ . " confocal microscopy" . . "0"^^ . "KJB5039302" . "6"^^ . "mesonephric vascular stream development; erythropoiesis; chick embryo; 1,2-dibromoethane; computerized image analysis; confocal microscopy; stereological methods"@en . . . "6"^^ . . . " erythropoiesis" . . . .