"2006-06-19+02:00"^^ . . "Optimalization of deep burns healing by application of human keratinocytes cultured on sterile porcine xenomatrix. Production of xenomatrix, which can be used alone as bio-cover, is being transferred to GEL-MED INTERNATIONAL Ltd."@en . . "3"^^ . "0"^^ . "3"^^ . "human keratinocyte cultivation" . "Optimalizace hojen\u00ED hlubok\u00FDch derm\u00E1ln\u00EDch pop\u00E1lenin lidsk\u00FDmi keratinocyty kultivan\u00FDmi na steriln\u00ED prase\u010D\u00ED xenomatrix. V\u00FDrobu xenomatrix, kter\u00E1 je pou\u017Eiteln\u00E1 i jako samostatn\u00FD kryt, p\u0159eb\u00EDr\u00E1 firma GEL-MED INTERNATIONAL, spol. s.r.o."@cs . "2"^^ . "recombined human/pig skin" . "xenodemis" . "IBS5052312" . "recombined human/pig skin;deep dermal burns;full thickness skin loss;skin substitute;xenodemis;human keratinocyte cultivation;prevention of conversion;wound management;wound preparation"@en . "1"^^ . . . . . "full thickness skin loss" . . "We introduced cultivation of human keratinocytes for treatment of burns in collaboratio between the IMG AS CR and the Prague Burn Centre. Later, a recombined artificial skin was developed. It consisted of human keratinocytes cultured on processed pig xenodermis. The application of the artificial skin with keratinocytes facing the wound resulted in nearly ideal bio-dressing stimulating healing of burns and donor areas and simultaneously reducint the risk of hypertrophic scarring. Using the bio-dressing with special wound managment achieved by gentle elimination of necrotic tissue a new approach to prevention of spontaneous conversion of deep 2nd degree into 3rd degree burns was established. The importance of the new treatmentis stressed by difficult diagnosis of deep dermal burns and need of autotransplation for healing the 3rd degree burns. For further clinical application the preparation of xenodermis, regularity of cultivation, and wound managment have to be optimized."@en . "prevention of conversion" . "http://www.isvav.cz/projectDetail.do?rowId=IBS5052312"^^ . . . . . "wound management" . . . . . . "Ve spolupr\u00E1ci \u00DAMG AV \u010CR a Kliniky pop\u00E1lenin FNKV v Praze 10 byla zavedena kultivace lidsk\u00FDch keratinocyt\u016F in vitro pro l\u00E9\u010Dbu pop\u00E1lenin. N\u00E1sledn\u011B byl origin\u00E1ln\u011B vy\u0159e\u0161en probl\u00E9m n\u00E1hrady derm\u00E1ln\u00ED slo\u017Eky um\u011Bl\u00E9 k\u016F\u017Ee pou\u017Eit\u00EDm vypreparovan\u00E9 prase\u010D\u00ED xenodermis. Inverzn\u00ED aplikac\u00ED um\u011Bl\u00E9 k\u016F\u017Ee byl z\u00EDsk\u00E1n t\u00E9m\u011B\u0159 ide\u00E1ln\u00ED bio-obvaz, kter\u00FD urychloval hojen\u00ED pop\u00E1lenin i odb\u011Brov\u00FDch ploch a z\u00E1rove\u0148 sni\u017Eoval riziko tvorby hypertrofick\u00FDch jizev. Kombinac\u00ED bio-obvazu a specieln\u00ED p\u0159\u00EDpravy r\u00E1ny \u0161etrn\u00FDm odstran\u011Bn\u00EDm nekr\u00F3zy se z\u00EDskal nov\u00FD l\u00E9\u010Debn\u00FD postup pro prevenci spont\u00E1nn\u00EDho a obt\u00ED\u017En\u011B diagnostikovateln\u00E9ho zvratu hlubok\u00E9 derm\u00E1ln\u00ED pop\u00E1leniny 2b na obt\u00ED\u017En\u011B l\u00E9\u010Diteln\u00FD 3. stupe\u0148 (l\u00E9\u010Diteln\u00FD jen autotransplantac\u00ED). P\u0159ed p\u0159ed\u00E1n\u00EDm tohoto postupu do klinick\u00E9ho ov\u011B\u0159ov\u00E1n\u00ED je t\u0159eba optimalizovat jeho jednotliv\u00E9 slo\u017Eky: preparaci xenodermis, spolehlivost i bezpe\u010Dnost in vitro tvorby um\u011Bl\u00E9 k\u016F\u017Ee a zp\u016Fsoby p\u0159\u00EDpravy r\u00E1ny p\u0159ed aplikac\u00ED bio-obvazu. Tento pot\u0159ebn\u00FD v\u00FDvoj a v\u00FDzkum je p\u0159edm\u011Btem navrhovan\u00E9ho projektu." . "deep dermal burns" . . . "Optimalizace aplikace um\u011Bl\u00E9 rekombinovan\u00E9 k\u016F\u017Ee pro l\u00E9\u010Debnou prevenci konverze hlubok\u00FDch derm\u00E1ln\u00EDch pop\u00E1lenin 2b stupn\u011B na 3. stupe\u0148" . . "Optimization of application of artificial recombined skin for preventing conversion of deep dermal to full thickness burns"@en . . "skin substitute" . .