"development of DNA hybridization sensors;electrochemical detection of DNA hybridization;electrochemistry of nucleic acids;mercury and solid electrodes;electrochemical analysis in genomics;nucleotide sequences in DNA and RNA"@en . . . "Elektroaktivita nukleov\u00FDch kyselin byla objevena navrhovatelem projektu asi p\u0159ed 40-ti lety. Velk\u00E9 mno\u017Estv\u00ED v\u011Bdomost\u00ED, zku\u0161enost\u00ED a KNOW-HOW, kter\u00E9 se nahromadily v jeho laborato\u0159i v uplynul\u00E9 dob\u011B, bude nyn\u00ED zam\u011B\u0159eno na z\u00E1kladn\u00ED a orientovan\u00FD v\u00FDzkum za \u00FA\u010Delem vytvo\u0159en\u00ED dobr\u00E9ho z\u00E1kladu pro v\u00FDvoj senzor\u016F hybridizace DNA. Interakce nukleov\u00FDch kyselin a modelov\u00FDch slou\u010Denin s elektrodami bude studov\u00E1na za \u00FA\u010Delem nalezen\u00ED a charakterizov\u00E1n\u00ED t\u011Bch proces\u016F na elektrod\u00E1ch, kter\u00E9 jsou nejvhodn\u011Bj\u0161\u00ED pro citlivou detekci hybridizace. Speci\u00E1ln\u00ED pozornost bude v\u011Bnov\u00E1na katalytick\u00FDm proces\u016Fm, kter\u00E9 maj\u00ED velk\u00FD elektronov\u00FD v\u00FDt\u011B\u017Eek. Hybridizace DNA bude zkoum\u00E1na nejen na elektrod\u00E1ch, ale i na jin\u00FDch povr\u0161\u00EDch, aby byla eliminov\u00E1na nebo sn\u00ED\u017Eena nespecifick\u00E1 adsorpce DNA a zv\u00FD\u0161ila se specificita hybridizace DNA na povrchu. Nov\u00E9 p\u0159\u00EDstupy jsou pot\u0159ebn\u00E9 (i) pro p\u0159ekon\u00E1n\u00ED probl\u00E9m\u016F v anal\u00FDze dlouh\u00FDch c\u00EDlov\u00FDch DNA, a (ii) pro v\u00FDvoj levn\u00FDch elektrochemick\u00FDch za\u0159\u00EDzen\u00ED s rychlou odezvou pro diagnostiku DNA." . "http://www.isvav.cz/projectDetail.do?rowId=IBS5004355"^^ . "Pro levnou anal\u00FDzu DNA byly navr\u017Eeny nov\u00E9 elektrody a elektrochemick\u00E9 metody zahrnuj\u00EDc\u00ED (i) modifikaci DNA elektroaktivn\u00EDmi zna\u010Dkami, (ii) stanoven\u00ED bodov\u00FDch mutac\u00ED v DNA pomoc\u00ED proteinu MutS, (iii) stanoven\u00ED d\u00E9lky dlouh\u00FDch repetitivn\u00EDch sekvenc\u00ED."@cs . "electrochemistry of nucleic acids" . . "Possibilities of electrochemical methods in genomics. Basis for development of DNA sensors."@en . . "mercury and solid electrodes" . . "IBS5004355" . . "2006-06-19+02:00"^^ . . . . "11"^^ . "11"^^ . . . . "1"^^ . . "electrochemical analysis in genomics" . . "Electroactivity of nucleic acids was discovered by the principal investigator about 40 yers ago. Great amount of knowledge, experience and know-how which accumulated in his laboratory during the past time will be now concentrated to the basic and oriented research to create a solid base for the development of DNA hybridization sensors. Interactions of nucleic acids and model compounds with electrodes will be studied to find and characterize electrode processes best suited for the sensitive detection of the hybridization event. Special attention will be devoted to high electron yield catalytic processes. DNA hybridization at different surfaces will be investigated to eliminate or decrease non-specific adsorption of DNA and improve the specificity of the surface DNA hybridization. New approaches will be sought (i) to overcome the difficultuies in the analysis of long target DNAs, and (ii) to develop low-cost, fast response electrochemical devices for the DNA diagnostics."@en . . "0"^^ . . "Mo\u017Enosti elektrochemick\u00FDch metod v genomice, z\u00E1klady v\u00FDvoje biosenzor\u016F DNA" . . . "0"^^ . "electrochemical detection of DNA hybridization" . . . "For inexpensive DNA analysis new electrodes and electrochemical methods: (i)DNA labeling with electroactive markers, (ii)determination of DNA point mutations using MutS protein, (iii)determination of lengths of long repetitive DNA sequences were proposed"@en . . "development of DNA hybridization sensors" .