. "2D kondenzace baz\u00ED a nukleosid\u016F nast\u00E1v\u00E1 tak\u00E9 na pevn\u00FDch polykrystalick\u00FDch amalgamov\u00FDch povr\u0161\u00EDch. Elektrochemick\u00E1 metoda umo\u017E\u0148uje citlivou detekci d\u00E9lky oligonukleotid\u016F. Byly pops\u00E1ny vlastnosti DNA modifikovan\u00E9 analogy protin\u00E1dorov\u011B \u00FA\u010Dinn\u00E9 cisplatiny."@cs . . "DNA absorption" . "electrochemical impedance" . "DNA damage" . . . "cytostatics" . . "Bude rozv\u00EDjena spolupr\u00E1ce t\u0159\u00ED laborato\u0159\u00ED BF\u00DA AV \u010CR, kter\u00E1 bude vych\u00E1zet z poznatk\u016F fyzik\u00E1ln\u00EDch princip\u016F interakc\u00ED nukleov\u00FDch kyselin a modelov\u00FDch slou\u010Denin s povrchy kov\u016F a s ligandy (n\u00E1dorov\u00FD supresor p53, cytostatika). Spolupr\u00E1ce bude sm\u011B\u0159ovat jednak ke konstrukci biosenzoru pro detekci po\u0161kozen\u00ED DNA a pro detekci jej\u00ED vazby s ligandy, jednak k v\u00FDvoji nov\u00FDch cytostatik na basi cis-platiny, kter\u00E1 budou m\u00EDt lep\u0161\u00ED protin\u00E1dorovou aktivitu a sn\u00ED\u017Eenou mutagenn\u00ED \u010Dinnost. Nov\u00E9 v\u00FDsledky p\u0159inese zejm\u00E9na propojen\u00ED v\u00FDzkumu na \u00FArovni fyzik\u00E1ln\u00ED, elektrochemick\u00E9 a molekul\u00E1rn\u011B biologick\u00E9. Projekt je zalo\u017Een na rozvinut\u00E9 dom\u00E1c\u00ED i zahrani\u010Dn\u00ED spolupr\u00E1ci, jej\u00EDm\u017E v\u00FDsledkem je \u0159ada publikac\u00ED v presti\u017En\u00EDch mezin\u00E1rodn\u00EDch \u010Dasopisech. Aktu\u00E1lnost navrhovan\u00E9 problematiky vypl\u00FDv\u00E1 ze z\u00E1jmu o p\u0159\u00EDm\u00E9 zapojen\u00ED v\u00FDrobn\u00EDch podnik\u016F v projektu." . "electrode double layer" . "The cooperation between three laboratories of the Institute of Biophysics proceeding from the knowledge of the physical principles of interactions of nucleis acids and model compounds with metal surfaces and with ligands (tumor supresor p53, cytostatics) will be further developed. The cooperation will be aimed on the one hand to the development of a biosensor for detection of DNA damage and detection of the binding of ligand to DNA, on the other hand to the development of new cytostatics based on cis-Pt with a higher antitumor activity and lower mutagenic effect. New results can be expected especially due to the interconnection of the research at the physical, electrochemical and molecular biological levels, leading to publications in high quality international journals. The subject of the proposed project is up-to-date as it follows from the interests of manufacturers and their direct involvement in the project."@en . "2006-06-19+02:00"^^ . "voltammetry" . . . "p53 protein" . "supercolied DNA" . . "Application of biophysical methods in biotechnological and clinical praxis"@en . . . . "tumor suppressor" . "http://www.isvav.cz/projectDetail.do?rowId=IBS5004107"^^ . . "0"^^ . "Aplikace biofyzik\u00E1ln\u00EDch metod v biotechnologick\u00E9 a klinick\u00E9 praxi" . . "DNA absorption;two-dimensional condensation;electrode double layer;electrochemical impedance;voltammetry;p53 protein;tumor suppressor;cytostatics;cisplatin;biosensor;DNA damage;DNA interactions;supercolied DNA;superhelix density"@en . . "1"^^ . . . . . "2D condensation of bases and nucleosides takes place at solid polycrystalline amalgam surfaces. Electrochemical method enables sensitive detection of oligonucleotides length. Properties of DNA modified by antitumor analogues of cisplatinum were described"@en . "IBS5004107" . "biosensor" . . . . "0"^^ . "two-dimensional condensation" . "DNA interactions" . "cisplatin" . "46"^^ . "46"^^ .