. "confocal microscopy" . "cytometry" . "The project is directed to the improvement of diagnostic techniques in oncology both in primary diseases and in related infections. The development of high-resolution cytometric techniques and methods of the detection of the telomerase activity will be the goals of the project. Topographic characteristics of appropriate genetic loci in selected tumour tissues will be mesured and correlated to tumour characteristcs. The development of techniques and methods for clinical applications will be followed by industrial development of a confocal cytometr. In addition, methods of detection of the telomerase activity and expresion will be optimised both for the diagnostic purposes, and for testing the sensitivity of cultured tumor cells to cytostatics and potential cytostatic agents. The improvement of diagnostic techniques of fungal infections typical of oncological patients will be the additional goal of the project. DNA probes will be prepared for clinica diagnostics."@en . . "telomerase" . . "Projekt je sm\u011B\u0159ov\u00E1n na zlep\u0161en\u00ED diagnostiky v onkologick\u00E9 praxi, a to jednak u prim\u00E1rn\u00EDho onemocn\u011Bn\u00ED a jednak u p\u0159idru\u017Een\u00FDch infekc\u00ED. C\u00EDlem projektu bude zejm\u00E9na v\u00FDvoj cytometrick\u00FDch technik s vysok\u00FDm rozli\u0161en\u00EDm a metod detekce telomerazov\u00E9 aktivity. Budou m\u011B\u0159eny topografick\u00E9 charakteristiky vhodn\u00FDch genetick\u00FDch oblast\u00ED ve vybran\u00FDch n\u00E1dorov\u00FDch tk\u00E1n\u00EDch a porovn\u00E1v\u00E1ny s vlasnostmi n\u00E1doru. Na c\u00EDlen\u00FD v\u00FDzkum v klinice bude navazovat pr\u016Fmyslov\u00FD v\u00FDvoj levn\u00E9ho konfok\u00E1ln\u00EDho cytometru. Metody detekce aktivity a exprese telomer\u00E1zy ve vzorc\u00EDch n\u00E1dorov\u00FDch tk\u00E1n\u00ED budou optimalizov\u00E1ny jednak pro \u00FA\u010Dely onkologick\u00E9 diagnostiky, jednak pro zji\u0161\u0165ov\u00E1n\u00ED citlivosti kultivovan\u00FDch n\u00E1dorov\u00FDch bun\u011Bk k cytostatik\u016Fm a potenci\u00E1ln\u00EDm cytostatik\u016Fm. Dal\u0161\u00EDm c\u00EDlem projektu bude zlep\u0161en\u00ED diagnostiky modern\u00EDch metod molekul\u00E1rn\u00ED biofyziky. Vedlej\u0161\u00EDm produktem v\u00FDzkumu budou DNA sondy pro diagnostiku malign\u00EDch onemocn\u011Bn\u00ED." . . "IBS5004010" . . "77"^^ . . . "molecular diagnostics" . "77"^^ . . "carcinogenesis" . "molecular diagnostics;cytometry;confocal microscopy;carcinogenesis;telomerase;mycoses"@en . "http://www.isvav.cz/projectDetail.do?rowId=IBS5004010"^^ . . . . "1"^^ . . . "1"^^ . "New results on the relation between the genome arrangement and its expression, on changes of the structure during cell differentiation and malignant transformation and on mechanisms of the regulation of the telomere length and telomerase were obtained."@en . "Development of new diagnostic techniques for oncology"@en . "2005-06-17+02:00"^^ . . . "0"^^ . . . . "V\u00FDvoj nov\u00FDch diagnostick\u00FDch technik pro onkologii" . . "Byly z\u00EDsk\u00E1ny nov\u00E9 origin\u00E1ln\u00ED poznatky o vztahu prostorov\u00E9ho uspo\u0159\u00E1d\u00E1n\u00ED genomu bu\u0148ky k jeho genov\u00E9 expresi, o zm\u011Bn\u00E1ch struktury a exprese v pr\u016Fb\u011Bhu diferenciace bun\u011Bk a malign\u00ED transformace a o mechanismech regulace d\u00E9lky telomer a aktivity telomer\u00E1zy."@cs . .