. . "Semisynthetick\u00E1 konstrukce v\u00FD\u0161emolekul\u00E1rn\u00EDch forem mal\u00FDch biologicky aktivn\u00EDch peptid\u016F a substr\u00E1t\u016F s desoktapeptidinsulinem" . "Deaoktapeptid insulin (DOI) je fragment insulinu, na kter\u00FD byly kovalentn\u011B p\u0159ipojeny biologicky aktivn\u00ED peptidy, substr\u00E1ty aminoeptidas a jin\u00E9 amino slou\u010Deniny. Byla vypracov\u00E1na metoda separace deaminovan\u00E9ho DOI (Asn A21--Asp A21) od nedeaminovan\u00E9ho."@cs . . "2006-06-19+02:00"^^ . "Derivatives of biologically active poptides with the prolonged action (precursors) are in some cases constructed as highmolecularinactive substances, which liberates the poptide concerned under conditions in vivo by the action of the specific hydrolytic enzymes. For example from Glypressin (triglycylvasopressin) vasopressin is released by aminopoptidases. By the tryptic treatment of porcine insulin inactive highmolecular fragment desoctapeptideinsulin (DOI) is formed. The same enzyme (trypsin) catalyzes in the partially non aqueous medium thy synthesis of peptide bond between carboxyl of B22 arginene adn a suitable peptide, analog of B23-B30 insulin sequence. A seiess ofnex semisynthetic analogs of insulin gave hope for the preparationof compounds of new type of compounds. DOI (biologically inactive insulin fragment) will be used for the construction of highmolecular precursors of small biol. active peptides which should under conditions in vivo liberates the peptide in time."@en . "Semisynthetic construction of high molecular forms of the small biologically active peptides and sebstrates with desoctapeptidiensulin"@en . . . . "0"^^ . . . "high molecular precursors" . . "1"^^ . "Desoctapeptide insulin (DOI) is the fragment of insulin to which the peptides, substrates of aminopeptidases and other amino substances were covalently bonded. The method of separation of deamidated DOI from the non-deamidated one was worked out."@en . . "2"^^ . . "Semisynthesis" . "0"^^ . . "2"^^ . "Prekursory, protrahovan\u011B p\u016Fsob\u00EDc\u00ED deriv\u00E1ty biologicky aktivn\u00EDch peptid\u016Fjsou v n\u011Bkter\u00FDch p\u0159\u00EDpadech konstruov\u00E1ny jako v\u00FD\u0161emolekul\u00E1rn\u00ED biologicky neaktivn\u00ED l\u00E1tky, ze kter\u00FDch se teprve v organismu, p\u016Fsoben\u00EDm hydrolytick\u00FDch enzym\u016F, uvol\u0148uj\u00ED postupn\u011B biologicky aktivn\u00ED peptidy. P\u0159\u00EDkladem m\u016F\u017Ee b\u00FDt triglycyl vasopresin (Glyprasin), ze kter\u00E9ho se p\u016Fsoben\u00EDm aminopeptidas generuje vasopresin. Tryptickou fragmentac\u00ED vep\u0159ov\u00E9ho insulinu vznik\u00E1 biologicky neaktivn\u00ED vysokomolekul\u00E1rn\u00ED desoktpeptid insulin (DOI), d\u00E1le heptapeptid a voln\u00FD alanin. V \u010D\u00E1sten\u011B nevhodn\u00E9m prost\u0159ed\u00ED v\u0161ak trypsin katalyzuje kondensa\u010Dn\u00ED reakci aminokyselin a peptid\u016F s karboxylem argininu v posici B23 DOI. P\u0159\u00EDprava a studium semisynthetick\u00FDch analog\u016F insulinu uk\u00E1zala sch\u016Fdnost tohoto p\u0159\u00EDstupu pro p\u0159\u00EDpravu nov\u00FDch l\u00E1tek. DOI (biologicky neaktivn\u00ED fragment) chceme v na\u0161em p\u0159\u00EDpad\u011B vyu\u017E\u00EDt pro konstrukce v\u00FD\u0161emolekul\u00E1rn\u00EDch prekursor\u016F mal\u00FDch biol. aktivn\u00EDch peptid\u016F, ze kter\u00FDch by se v \u010Dase uvol\u0148oval enzymov\u011B p\u0159\u00EDslu\u0161n\u00FD peptid." . "IBS4055303" . "desoctapeptideinsulin" . "biologically active peptides" . . . . "Semisynthesis;desoctapeptideinsulin;biologically active peptides;high molecular precursors;bioavailibity of peptides;enzymic conversion"@en . . "bioavailibity of peptides" . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=IBS4055303"^^ .