. "separation" . "A large number of biochemical and clinical applications requires analytical procedures with positive identification of the analyzed substances by mass spectrometry. Such applications include analyses of protein digests, high throughput purity control of drugs and drug candidates and/or analysis of physiological markers and metabolites. In all cases a high throughput (>100 samples/day) is typically required with the need for salt removal, enrichmentor separation. The focus of this research will be on development of microfabricated devidces for analysis of minute amounts of clinical samples with direct coupling to ESI/MS. An important part of the research will be also the design of an ESI interface with enhanced ion transmission into the MS suitable for direct coupling og the microdevices with the mass spectrometer. The microdevices, automatically positioned in front of the mass spectrometer, will have a capacity of up to hundred samples and will be dispossable."@en . . . "microfabrication" . . "0"^^ . "Velk\u00FD po\u010Det biochemick\u00FDch a klinick\u00FDch aplikac\u00ED vy\u017Eaduje analytick\u00E9 postupy s jednozna\u010Dnou identifikac\u00ED analyzovan\u00FDch l\u00E1tek hmotnostn\u00ED spektrometri\u00ED. Mezi takov\u00E9 aplikace pat\u0159\u00ED nap\u0159\u00EDklad anal\u00FDzy proteinov\u00FDch digest\u016F, anal\u00FDza \u010Distoty velk\u00FDch soubor\u016F vzork\u016F l\u00E9\u010Div a jejich kandid\u00E1t\u016F, nebo anal\u00FDza biologick\u00FDch marker\u016F a metabolit\u016F. V uveden\u00FDch p\u0159\u00EDkladech se obvykle jedn\u00E1 o velk\u00E9 po\u010Dty vzork\u016F (>100/den) \u010Dasto s nutnost\u00ED jejich odsolen\u00ED, p\u0159edkoncentrace, nebo separace. Z\u00E1m\u011Brem tohoto v\u00FDzkumu bude v\u00FDvoj mikrofabrikovan\u00FDch za\u0159\u00EDzen\u00ED pro anal\u00FDzy mal\u00FDch mno\u017Estv\u00ED klinick\u00FDch vzork\u016F s p\u0159\u00EDm\u00FDm napojen\u00EDm na ESI/MS. D\u016Fle\u017Eitou sou\u010D\u00E1st\u00ED v\u00FDzkumu bude tak\u00E9 n\u00E1vrh ESI rozhran\u00ED se zv\u00FD\u0161enou iontovou propustnost\u00ED pro p\u0159\u00EDm\u00E9 p\u0159ipojen\u00ED mikroza\u0159\u00EDzen\u00ED k hmotnostn\u00EDmu spektrometru. Mikro\u010Dipy s kapacitou a\u017E sto vzork\u016F budou automaticky polohov\u00E1ny p\u0159ed hmotnostn\u00EDm spektromerem a budou ur\u010Deny jen na jedno pou\u017Eit\u00ED (disposable)." . . "2"^^ . "Byly vyvinuty postupy p\u0159\u00EDpravy a pou\u017Eit\u00ED mikrofluidiky pro anal\u00FDzu biologick\u00FDch vzork\u016F a nov\u00E9 principy spojen\u00ED s hmotnostn\u00ED spektrometri\u00ED. Projekt p\u0159isp\u011Bl k vybudov\u00E1n\u00ED z\u00E1kladn\u00ED infrastruktury nov\u00E9ho odd\u011Blen\u00ED pro v\u00FDzkum bioanalytick\u00E9 mikrofluidiky."@cs . "1"^^ . . "Kombinace mikro\u010Dipu s hmotnostn\u00ED spektrometri\u00ED pro vysoce efektivn\u00ED popula\u010Dn\u00ED a selektivn\u00ED screening vrozen\u00FDch metabolick\u00FDch poruch" . . "selective screening" . "15"^^ . "MS/MS" . . . "15"^^ . . . "Coupling of microchip with mass spectrometry for high throughput population and selective screening of inborn metabolic disorders"@en . "microfabrication;microchip;separation;electrospray;MS/MS;metabolic disorders;selective screening;population screening"@en . . "2006-06-19+02:00"^^ . "The protocols for fabrication and use of microfluidics were established and new approaches for coupling with mass spectrometry were developed. Additionally, the research infrastructure for bioanalytical microfluidics in a new department was improved."@en . . . . . "microchip" . "electrospray" . "metabolic disorders" . "http://www.isvav.cz/projectDetail.do?rowId=IBS4031209"^^ . . . . . "IBS4031209" . .