" human granulocztes" . " in vitro myeolid differentiation" . . "2004-10-13+02:00"^^ . "Chromatin structure; territorial model of chromosomes; protooncogenes; FISH; RT/PCR; geneexpression; human granulocztes; in vitro myeolid differentiation; neukemic cells;"@en . . "Jadern\u00E1 topologie n\u011Bkter\u00FDch protoonkogen\u016F u lidsk\u00FDch neutrofiln\u00EDch granulocyt\u016F a leukemick\u00FDch bun\u011Bk" . . "http://www.isvav.cz/projectDetail.do?rowId=IAB5004102"^^ . "24"^^ . "24"^^ . " geneexpression" . "Pr\u00E1ce bude \u0159e\u0161it problematiku topologie C-MYC, ABL a BCR protookogen\u016F v j\u00E1drech lidsk\u00FDch granulocyt\u016F v porovn\u00E1n\u00ED s jadern\u011B topografick\u00FDmi parametry zm\u00EDn\u011Bn\u00FDch gen\u016F u leukemick\u00FDch bun\u011Bk, u kter\u00FDch bude navozena myeloidn\u00ED diferenciace, b\u011Bhem kter\u00E9 se m\u011Bn\u00ED exprese n\u011Bkter\u00FDch studovan\u00FDch gen\u016F. Bude hled\u00E1n vztah mezi strukturou jadern\u00E9ho chromatinu a expres\u00ED gen\u016F nejen v modelov\u00FDch p\u0159\u00EDkladech experiment\u00E1ln\u011B navozen\u00E9 diferenciace leukemick\u00FDch bun\u011Bk, ale i u pacient\u016F s chronickou myeloidn\u00ED leukemi\u00ED (CML). U pacient\u016F bude sledov\u00E1n rozd\u00EDl v topologii ABL a BCR gen\u016F v obdob\u00ED chronick\u00E9 a akcelera\u010Dn\u00ED f\u00E1ze a v obdob\u00ED blastick\u00E9ho zvratu, kdy se m\u011Bn\u00ED exprese ABL genu, kter\u00FD je u t\u00E9to formy leukemie translokov\u00E1n do oblasti BCR lokusu (Philadelphsk\u00FD chromosom). Rovn\u011B\u017E bude sledov\u00E1na amplifikace C-MYC genu. C\u00EDlem pr\u00E1ce je zjistit zda existuj\u00ED rozd\u00EDly v topografii studovan\u00FDch gen\u016F u pacient\u016F s CML v porovn\u00E1n\u00ED se zdrav\u00FDmi jedinci." . . " neukemic cells" . . . . . "Chromatin structure" . "IAB5004102" . "The project will study the topology of C-MYC, ABL and BCR protoocogenes in the nuclei of human granulocytes in comparison with the topology of mentioned genes in leukemic cells that will be differentiated along the myeloid pathway taht is characteristic by changes of gene expression. The relationship between the structure of nuclear chromatin and gene expression will be studied not only in the model differentiation experiments, but also in the parients suffering from chronic myelogenous leukemia (CML). The differences in the topology of ABL and BCR genes will be detected at the time of chronic, accelerated phases and at the time of blastic crisis of CML which differ in the expression of ABL gene that is translocated into the region of BCR locus (Philadelphia chromosome). Amplification of the C-MYC gene will be also assessed. The aim of this work is to find the differences in the topology of some genes that will be detected in the CML patients in contrast with the healthy individuals."@en . " territorial model of chromosomes" . . . . . " RT/PCR" . "Nuclear topology of some protooncogenes in human neutrophilic granulocytes and leukemic cells"@en . . . . "0"^^ . . "0"^^ . . "2"^^ . " protooncogenes" . " FISH" . "Granulocyt\u00E1rn\u00ED direrenciace bun+k je charakteristick\u00E1 repozic\u00ED \u0159ady genetick\u00FDch struktur k jadern\u00E9 membr\u00E1n\u011B. Existuj\u00ED z\u00E1vislosti mezi aktivitou/inaktivitou C-myc, Ab1 a Rb1 gen\u016F a p\u0159ibl\u00ED\u017Een\u00EDm t\u011Bchto lokus\u016F k centromerick\u00E9mu heterochromatinu."@cs .