"1"^^ . . . . "0"^^ . "Ultrastructural Study of Specific Features of RNA Kinetics in Mammalian Oocytes at Final Stages of Folliculogenesis"@en . "Ultrastruktur\u00E1ln\u00ED studie specifick\u00FDch rys\u016F kinetiky RNA v oocytech savc\u016F v kone\u010Dn\u00E9 f\u00E1zi folikulogenese" . . . . . "11"^^ . . "11"^^ . "http://www.isvav.cz/projectDetail.do?rowId=IAA752403"^^ . "Vliv oocyt\u016F na v\u00FDvoj a diferenciaci embrya je zprost\u0159edkov\u00E1n z\u00E1sobou makromolekul mate\u0159sk\u00E9ho p\u016Fvodu. V p\u0159edlo\u017Een\u00E9m projektu se proto chceme zab\u00FDvat (s vyu\u017Eit\u00EDm unik\u00E1tn\u00EDch metodick\u00FDch mo\u017Enost\u00ED z\u016F\u010Dastn\u011Bn\u00FDch pracovi\u0161\u0165) topologickou dynamikou ribonukleov\u00FDch kyselin a jejich specifick\u00FDch doprovodn\u00FDch protein\u016F, tj. nejd\u016Fle\u017Eit\u011Bj\u0161\u00EDch slo\u017Eek mate\u0159sk\u00E9ho p\u016Fvodu. Pro detekci transkrip\u010Dn\u00EDch m\u00EDst a distribuce RNA v kompartmentech j\u00E1dra vyu\u017Eijeme ultrastruktur\u00E1ln\u00ED neizotopovou metodu zalo\u017Eenou na mikroinjekci 5-bromouridinu-5'-trifosf\u00E1tu do oocyt\u016F. Mikroinjek\u010Dn\u00ED techniku d\u00E1le vyu\u017Eijeme p\u0159i aplikaci reagenci\u00ED pou\u017Eiteln\u00FDch k imunoelektronoptick\u00E9 detekci RNP. Poznatky o vnitrobun\u011B\u010Dn\u00E9 kinetice RNA p\u0159isp\u011Bj\u00ED k obecn\u00E9mu pozn\u00E1n\u00ED diferenciace oocyt\u016F a poskytnou z\u00E1kladpro objektivn\u00ED hodnocen\u00ED v\u00FDvojov\u00E9ho potenci\u00E1lu oocyt\u016F a embry\u00ED." . "IAA752403" . . . . . "1"^^ . .