"Protein-carbohydrate interactions are the example of noncovalent type of interactions which are taking place during transmission of infective agents by vectors on the molecular level. They represent the important aspect of molecular ecology of vector transmitted infections. Project deals with the study of carbohydrate binding proteins (lectins) in transmission system of the tisk Ixodes ricinus - Borrelia burgdorferi sl in respect to processes occured in hemocoel of the infected ticks. Project is aimed at isolation and purification of lectins from the tick hemolymph and borrelia spirochetes, at determination of whole sequences of their genes, at preparation of recombinant molecules of lectins, and their functional and structural characterization. An influence of plasma lectin and other protein fractions of hemolymph, midgut and salivary glands on development of borrelia in vitro from the point of employment of protein-carbohydrate interactions will be addressed."@en . . . "2"^^ . . . "Vybran\u00E9 aspekty molekul\u00E1rn\u00ED ekologie Lymesk\u00E9 borreli\u00F3zy" . "0"^^ . "5"^^ . " borrelia" . "Byl izolov\u00E1n a charakterizov\u00E1n lektin LECB31 z povrchu spiroch=t Lymesk\u00E9 borreli\u00F3zy, jako\u017E i jeho k\u00F3duj\u00EDc\u00ED gen. P\u0159ipraven byl rovn\u011B\u017E v rekombinantn\u00ED podob\u011B. Plasmov\u00FD lektin Dorin M byl izolov\u00E1n z hemolymfy kl\u00ED\u0161t\u011Bte O. moubata a lokalizov\u00E1n v hemocytech."@cs . . " glycans" . . . . " lectin-carbohydrate interaction" . " Molecular ecology" . . "5"^^ . "Lyme borreliosis; Molecular ecology; borrelia; lectin-carbohydrate interaction; recombinant lectin; glycans;"@en . "Interakce typu protein-sacharid jsou p\u0159\u00EDkladem nekovalentn\u00EDch interakc\u00ED, kter\u00E9 se uplat\u0148uj\u00ED p\u0159i p\u0159enosu p\u016Fvodc\u016F n\u00E1kaz vektory na molekulov\u00E9 \u00FArovni. P\u0159edstavuj\u00ED tak v\u00FDznamn\u00FD aspekt molekul\u00E1rn\u00ED ekologie vektory p\u0159en\u00E1\u0161en\u00FDch n\u00E1kaz. Projekt se zab\u00FDv\u00E1 studiem protein\u016F v\u00E1zaj\u00EDc\u00EDch sacharidy (lektin\u016F) v modelov\u00E9m syst\u00E9mu kl\u00ED\u0161t\u011B Ixodes ricinus-spirocheta Borrelia burgdorferi s ohledem k d\u011Bj\u016Fm v hemocelu infikovan\u00FDch kl\u00ED\u0161\u0165at. Zad\u00E1n\u00ED projektu je soust\u0159ed\u011Bno na izolaci a purifikaci lektinu z hemolymfy kl\u00ED\u0161\u0165at a povrchu borr\u00E9li\u00ED, ur\u010Den\u00ED \u00FApln\u00E9 sekvence jejich gen\u016F, p\u0159\u00EDpravu rekombinan\u010Dn\u00EDch molekul lektin\u016F a jejich funk\u010Dn\u011B strukturn\u00ED charakterizace. Analyzov\u00E1ny budou \u00FA\u010Dinky plazmov\u00E9ho lektinu a dal\u0161\u00EDch proteinov\u00FDch frakc\u00ED hemolymfy, nesenteronu a slinn\u00FDch \u017El\u00E1z nav\u00FDvoj borr\u00E9li\u00ED in vitro z hlediska uplatn\u011Bn\u00ED protein-sacharidov\u00FDch interakc\u00ED." . . . . . "2004-10-27+02:00"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=IAA6022001"^^ . "Lyme borreliosis" . "IAA6022001" . . " recombinant lectin" . "The selected aspects of molecular ecology of Lyme borreliosis"@en . . . "0"^^ .