"2010-12-31+01:00"^^ . . " saliva activated transmission" . . . . "Identification of tick salivary molecules facilitating transmission of Borrelia burgdorferi using RNA interference"@en . " Borrelia burgdorferi" . . "9"^^ . . "9"^^ . "ticks" . "http://www.isvav.cz/projectDetail.do?rowId=IAA600960811"^^ . . "2010-03-09+01:00"^^ . "IAA600960811" . . . "Tick-born pathogens exploit tick saliva for successful transmission and proliferation in the host. This phenomenon is called saliva-activated transmission (SAT). Although several candidate SAT factors have been proposed, only one tick salivary protein (Salp 15) facilitating infection of the host with Borrelia spirochetes has been identified. It is very likely that immunomodulatory (immunosuppressive) molecules in tick saliva are responsible for SAT effect. The main objective is to identify SAT molecules by gene silencing using RNA interference. Genes coding for secreted proteins inducible by tick feeding will be selected from cDNA libraries prepared from tick salivary glands. Ticks with silenced genes will be tested for the ability to accelerate proliferation of Borrelia spirochetes in the host skin. Recombinant proteins will be produced and tested for the SAT and immunomodulatory activity. The research will be performed using Ixodes ricinus ticks and Borrelia burgdorferi s.s."@en . . "ticks; saliva activated transmission; Borrelia burgdorferi; RNA interference"@en . . . "0"^^ . "0"^^ . "2013-06-28+02:00"^^ . . "1"^^ . . "Sialostatin L2, facilitating transmission of Borrelia spirochetes by ticks, has been identified in tick saliva. Moreover, other proteins with antihaemostatic and immunomodulatory effect and vaccination and antiallergic potential have been discovered."@en . "2008-01-01+01:00"^^ . . "V kl\u00ED\u0161t\u011Bc\u00EDch slin\u00E1ch byl identifikov\u00E1n protein usnad\u0148uj\u00EDc\u00ED p\u0159enos boreli\u00ED kl\u00ED\u0161\u0165aty, sialostatin L2. Krom\u011B toho byly identifikov\u00E1ny dal\u0161\u00ED proteiny s antihemostatickou a imunomodula\u010Dn\u00ED aktivitou s vakcina\u010Dn\u00EDm a antialergick\u00FDm potenci\u00E1lem."@cs . "Identifikace molekul kl\u00ED\u0161t\u011Bc\u00EDch slin usnad\u0148uj\u00EDc\u00EDch p\u0159enos Borrelia burgdorferi pomoc\u00ED RNA interference" . . "Patogeny p\u0159en\u00E1\u0161en\u00E9 kl\u00ED\u0161\u0165aty vyu\u017E\u00EDvaj\u00ED kl\u00ED\u0161t\u011Bc\u00ED sliny k \u00FAsp\u011B\u0161n\u00E9mu p\u0159enosu a proliferaci v hostiteli. Tento jev je ozna\u010Dov\u00E1n jako slinami aktivovan\u00FD p\u0159enos (SAT). A\u010Dkoli byla navr\u017Eena \u0159ada kandid\u00E1tn\u00EDch SAT faktor\u016F, byl identifikov\u00E1n pouze jedin\u00FD protein kl\u00ED\u0161t\u011Bc\u00EDch slin (Salp 15), kter\u00FD usnad\u0148uje infekci hostitele boreliemi. Je velmi pravd\u011Bpodobn\u00E9, \u017Ee za SAT efekt jsou zodpov\u011Bdn\u00E9 imunomodula\u010Dn\u00ED (imunosupresivn\u00ED) molekuly kl\u00ED\u0161t\u011Bc\u00EDch slin. Hlavn\u00EDm c\u00EDlem projektu je identifikovat SAT molekuly vyp\u00EDn\u00E1n\u00EDm gen\u016F metodou RNA interference. Geny k\u00F3duj\u00EDc\u00ED sekretovan\u00E9 proteiny indukovan\u00E9 s\u00E1n\u00EDm krve budou vybr\u00E1ny z cDNA knihoven p\u0159ipraven\u00FDch ze slinn\u00FDch \u017El\u00E1z. Kl\u00ED\u0161\u0165ata s vypnut\u00FDmi geny budou testov\u00E1na na schopnost zvy\u0161ovat proliferaci boreli\u00ED v k\u016F\u017Ei hostitele sv\u00FDmi slinami. Na z\u00E1klad\u011B z\u00EDskan\u00FDch informac\u00ED budou produkov\u00E1ny rekombinantn\u00ED proteiny a bude zhodnocena jejich imunomodula\u010Dn\u00ED aktivita a aktivita v SAT testu. K v\u00FDzkumu budou pou\u017Eita kl\u00ED\u0161\u0165ata Ixodes ricinus a spirochety Borrelia burgdorferi s.s." . . .