. "2010-03-09+01:00"^^ . "Cytokinins (CKs) are known to act in combination with other plant hormones to regulate diverse developmental processes in plants. The exact molecular mechanisms of CK action in regulation of plant development are the subject of intense current research. While genome expression profiling of CK action has already been initiated by other groups elsewhere, here we propose to analyze proteome dynamics in response to increased CK levels in Arabidopsis under conditions similar to those used in monitoring genome-wide changes in gene expression. This will allow, for the first time, a comparison of the transcriptome and proteome data on highly comparable samples. Increase in CK levels will be achieved by treatment with exogenous CKs and by employing a novel dexamethasone-inducible gene expression system pOp/LhGR. A direct comparison of effects of three distinct CK classes is expected to contribute to elucidation of their individual function(s) in regulation of plant development."@en . " 2-D protein electrophoresis" . . . . "2010-12-31+01:00"^^ . . "IAA600040701" . . " proteome" . "Cytokininy (CK) se pod\u00EDlej\u00ED, v interakci s dal\u0161\u00EDmi rostlinn\u00FDmi hormony, na regulaci \u0159ady v\u00FDvojov\u00FDch proces\u016F u rostlin. P\u0159esn\u00E9 molekul\u00E1rn\u00ED mechanizmy p\u016Fsoben\u00ED CK v regulaci v\u00FDvoje rostlin jsou v sou\u010Dasnosti p\u0159edm\u011Btem intenzivn\u00EDho v\u00FDzkumu. Zat\u00EDmco anal\u00FDza profil\u016F exprese genomu v pr\u016Fb\u011Bhu p\u016Fsoben\u00ED CK byla ji\u017E zapo\u010Data jin\u00FDmi skupinami, zde navrhujeme analyzovat dynamiku proteomu v odpov\u011Bdi na zv\u00FD\u0161en\u00ED hladin CK u Arabidopsis za podm\u00EDnek podobn\u00FDch t\u011Bm, kter\u00E9 byly pou\u017Eity d\u0159\u00EDve p\u0159i sledov\u00E1n\u00ED zm\u011Bn v genov\u00E9 expresi na \u00FArovni cel\u00E9ho genomu. To n\u00E1m umo\u017En\u00ED prov\u00E9st prvn\u00ED srovn\u00E1n\u00ED \u00FAdaj\u016F popisuj\u00EDc\u00EDch transkriptom a proteom u vysoce podobn\u00FDch vzork\u016F. Zv\u00FD\u0161en\u00ED hladin CK bude dosa\u017Eeno p\u016Fsoben\u00EDm exogenn\u00EDch CK a vyu\u017Eit\u00EDm nov\u00E9ho syst\u00E9mu genov\u00E9 exprese indukovateln\u00E9 dexametazonem \u2013 pOp/LhGR. Lze o\u010Dek\u00E1vat, \u017Ee p\u0159\u00EDm\u00E9 srovn\u00E1n\u00ED \u00FA\u010Dink\u016F t\u0159\u00ED odli\u0161n\u00FDch t\u0159\u00EDd CK p\u0159isp\u011Bje k objasn\u011Bn\u00ED jejich individu\u00E1ln\u00EDch funkc\u00ED v regulaci v\u00FDvoje rostlin." . . "1"^^ . . . "cytokinins; proteome; protein phosphorylation; Arabidopsis; dexamethasone-inducible gene expression system; 2-D protein electrophoresis; MALDI-TOF/TOF MS"@en . "3"^^ . "Byly identifikov\u00E1ny proteiny a fosfoproteiny regulovan\u00E9 cytokininy u semen\u00E1\u010Dk\u016F Arabidopsis. Byla charakterizov\u00E1na dynamika jejich zm\u011Bn v odpov\u011Bdi na zv\u00FD\u0161en\u00E9 hladiny cytokinin\u016F za standardn\u00ED a sn\u00ED\u017Een\u00E9 intenzity sv\u011Btla."@cs . . . "0"^^ . "Cytokinin response proteins and phosphoproteins of Arabidopsis seedlings were identified, and their dynamics in response to increased cytokinin levels was characterized at standard and decreased intensity of white light."@en . . . "Proteome dynamics in response to increased cytokinin levels in Arabidopsis"@en . . " dexamethasone-inducible gene expression system" . " protein phosphorylation" . "Dynamika proteomu v odpov\u011Bdi na zv\u00FD\u0161en\u00E9 hladiny cytokinin\u016F u Arabidopsis" . "13"^^ . "13"^^ . " Arabidopsis" . "2013-06-28+02:00"^^ . "cytokinins" . . "2007-01-01+01:00"^^ . . . . "http://www.isvav.cz/projectDetail.do?rowId=IAA600040701"^^ . . .