. . . . "2009-12-31+01:00"^^ . "IAA600040505" . . "Activation of alternative telomere lengthening was shown in plants lacking telomerase activity. Plant telomerases producing human-type-telomeres were characterised. Tel. proteins and interactions were described in plants with typical or alt. telomeres."@en . . . . "Mechanismy tvorby a ztr\u00E1ty telomer nez\u00E1visl\u00E9 na telomer\u00E1ze" . . . . "Rostlinn\u00E1 \u010Dele\u010F Alliaceae se vyzna\u010Duje zna\u010Dnou variabilitou telomer. Zat\u00EDmco u \u0159ady rod\u016F t\u00E9to \u010Deledi jsou tzv. typick\u00E9 rostlinn\u00E9 telomery o sekvenci [TTTAGGG]n nahrazeny telomerami lidsk\u00E9ho typu [TTAGGG]n a dal\u0161\u00EDmi variantn\u00EDmi motivy ([TTAGG]n, [TTGGGG]n), u dal\u0161\u00EDch z\u00E1stupc\u016F, \u0159azen\u00FDch zejm. do rodu Allium, zcela chyb\u00ED jak\u00E9koli zn\u00E1m\u00E9 minisatelitn\u00ED telomerov\u00E9 sekvence. A\u010Dkoli bylo navr\u017Eeno, \u017Ee telomerizaci u t\u011Bchto druh\u016F zabezpe\u010Duj\u00ED satelitn\u00ED repetice nebo mobiln\u00ED elementy sv\u00FDmi amplifika\u010Dn\u00EDmi mechanismy, termin\u00E1ln\u00ED sekvence chromoz\u00F3m\u016F nebyly detailn\u011B charakterizov\u00E1ny. C\u00EDlem navrhovan\u00E9ho projektu je proto prov\u00E9st charakterizaci t\u011Bchto sekvenc\u00ED a jejich nukleoproteinov\u00FDch komplex\u016F a detekovat intermedi\u00E1ty procesu amplifikace t\u011Bchto sekvenc\u00ED, event. opa\u010Dn\u00E9ho procesu zvan\u00E9ho rychl\u00E1 ztr\u00E1ta telomer. Pracovn\u00ED hypot\u00E9za je zalo\u017Eena na p\u0159edpokladu, \u017Ee nejstar\u0161\u00EDm a evolu\u010Dn\u011B konzervovan\u00FDm mechanismem telomerizace line\u00E1rn\u00EDch chromoz\u00F3m\u016F je telomerov\u00E1 smy\u010Dka, prok\u00E1zan\u00E1 u \u0159ady eukaryot." . "2009-04-28+02:00"^^ . "The plant family Alliaceae shows a remarkable variability of telomeres. While in some genera of this family the %22typical%22 plant telomers of (TTTAGGG)n sequence are substituted by human type of telomeres /TTAGGG)n and other variant motifs ([TTaGG]n,[TTGGG]n), in others (namely from the genus Allium) any known minisatellite telomere wequences are completely missing. Although it has been suggested that telomerisation in these species is ensured by satellite repeats or mobile elements by their amplification mechanisms, the terminal chromosome sequences have not been characterised in detail. The aim of the proposed project is therefore to characterise these sequences and their nucleoprotein complexes, and to detect intermediates of their amplification or of the reverse process called telomere rapid deletion. The working hypothesis is based on presumption that the oldest and evolutionary conserved mechanism of telomerisation is telomere loop present in a number of eukaryotes."@en . . "2005-01-01+01:00"^^ . " chromosomes" . . "0"^^ . " alternative telomere lengthening" . "1"^^ . "Telomerase-independent mechanismus of telomere synthesis and loss"@en . . . "plant telomeres; alternative telomere lengthening; chromatin structure; DNA; proteins; chromosomes; molecular analysis"@en . "48"^^ . "0"^^ . "48"^^ . "plant telomeres" . " proteins" . "2010-06-08+02:00"^^ . " DNA" . . . "http://www.isvav.cz/projectDetail.do?rowId=IAA600040505"^^ . " chromatin structure" . . "U rostlin po ztr\u00E1t\u011B aktivity telomer\u00E1zy byla prok\u00E1z\u00E1na aktivace alternativn\u00ED tvorby telomer. Byly charakterizov\u00E1ny rostl. telomer\u00E1zy syntetizuj\u00EDc\u00ED lidsk\u00FD typ telomer. U rostlin s typic. i alt. telomerami byly pops\u00E1ny tel. proteiny a jejich interakce."@cs .