. "1"^^ . "0"^^ . . . "1"^^ . "0"^^ . "Navrhujeme objasnit sign\u00E1ln\u00ED dr\u00E1hy \u0159\u00EDd\u00EDc\u00ED specifickou aktivaci tyrosin kin\u00E1z v my\u0161\u00EDch spermi\u00EDch b\u011Bhem fertilizace a identifikovat jejich jednotliv\u00E9 \u010Dleny, v\u010Detn\u011B t\u011Bch, kter\u00E9 p\u0159\u00EDmo interaguj\u00ED s receptorem pro z\u00F3nu pelucidu. Vyu\u017Eijeme p\u0159itom jednak na\u0161ich origin\u00E1ln\u00EDch pozorov\u00E1n\u00ED, n\u00E1mi p\u0159ipraven\u00FDch protil\u00E1tek a tak\u00E9 metod, kter\u00E9 vych\u00E1zej\u00ED z rozs\u00E1hl\u00FDch strukturn\u00EDch a funk\u010Dn\u00EDch vlastnost\u00ED ji\u017E zn\u00E1m\u00FDch tyrosinov\u00FDch kin\u00E1z, kter\u00E9 byly \u00FAsp\u011B\u0161n\u011B vyu\u017Eity v jin\u00FDch bun\u011B\u010Dn\u00FDch syst\u00E9mech. Na\u0161\u00ED v\u00FDhodou budou rovn\u011B\u017E detailn\u00ED zku\u0161enosti s tyrosin kin\u00E1zovou aktivitou v my\u0161\u00EDch sperimi\u00EDch z\u00EDskan\u00E9 p\u0159i ned\u00E1vn\u00E9 izolaci a identifikaci %22p95%22 proteinu, o n\u011Bm\u017E bylo p\u0159edpokl\u00E1d\u00E1no, \u017Ee je my\u0161\u00EDm spermiov\u00FDm receptorem ZP s asociovanou tyrosin kin\u00E1zovou aktivitou. Budeme vych\u00E1zet i izolace, sekvenov\u00E1n\u00ED a stanoven\u00ED vnitrobun\u011B\u010Dn\u00E9 lokalizace vybran\u00FDch tyrosin fosforylovan\u00FDch protein\u016F v ny\u0161\u00ED spermii, a d\u00E1le z charakterizace a identifikace odpov\u00EDdaj\u00EDc\u00EDch tyrosinov\u00FDch kin\u00E1z a stanoven\u00ED jej\u00EDch regula\u010Dn\u00EDch vlastnost\u00ED a lokalizace." . "The participation of tyrosine kinases in the signal transduction in mouse sperm during fertilization"@en . "http://www.isvav.cz/projectDetail.do?rowId=IAA545401"^^ . . . "\u00DA\u010Dast tyrosin kin\u00E1z v sign\u00E1ln\u00ED transdukci u my\u0161\u00EDch spermi\u00ED b\u011Bhem fertilizace" . . "IAA545401" . . . . . . . "0"^^ .