. . "http://www.isvav.cz/projectDetail.do?rowId=IAA520405"^^ . . . . . "IAA520405" . "5"^^ . . "Studium utilizace malt\u00F3zy a maltodextrin\u016F thermofiln\u00ED aktinomyc\u00E9tou Thermomonospora curvata" . "5"^^ . "Maltose and Maltodextrin Utilization By the Thermophilic Actinomycete Thermomonospora curvata"@en . . . . "V tomto projektu bychom se r\u00E1di uab\u00FDvali studiem vyu\u017E\u00EDv\u00E1n\u00ED \u0161krobu, maltodextrin\u016F a malt\u00F3zy thermofiln\u00ED aktinomyc\u00E9tou Thermomonospora curvata, aglA, gen k\u00F3duj\u00EDc\u00ED alfa-glukosid\u00E1zu, a geny lokalizovan\u00E9 v jeho sousedstv\u00ED budou kompletn\u011B sekvencov\u00E1ny a d\u00E1le charakterizov\u00E1ny. Budou ur\u010Deny po\u010D\u00E1tky transkripce jednotliv\u00FDch gen\u016F a sledov\u00E1n efekt r\u016Fzn\u00FDch cukr\u016F na transkripci mRNA a na jej\u00ED stabilitu. Vlastnosti pravd\u011Bpodobn\u00E9ho regula\u010Dn\u00EDho proteinu budou zkoum\u00E1ny pomoc\u00ED in-vitro i in-vivo metod. Jeho funkce a vliv na expresi alva-amyl\u00E1zov\u00E9ho a alfa-glukosid\u00E1zov\u00E9ho genu bude prov\u011B\u0159ov\u00E1n v T. curvata a tak\u00E9 v hostitelsk\u00E9m kmeni Streptomyces. U purifikovan\u00E9ho proteinu bude testov\u00E1na DNA vazebn\u00E1 schopnost a budou ur\u010Dov\u00E1ny vazebn\u00E9 motivy na molekule DNA. Protil\u00E1tkyproti protein\u016Fm k\u00F3dovan\u00FDm ORF1 a ORF2 n\u00E1m umo\u017En\u00ED sledovat obsah protein\u016F v bu\u0148k\u00E1ch b\u011Bhem r\u016Fstu a dovol\u00ED n\u00E1m ur\u010Dit i jejich lokalizaci. V sou\u010Dasnosti tak\u00E9 pracujeme na zaveden\u00ED klonovac\u00EDho syst\u00E9mu v T. curvata" . "0"^^ . . "0"^^ . . . . "1"^^ .