. . " annexin II" . "P\u0159ib\u00FDvaj\u00EDc\u00ED \u00FAdaje podporuj\u00ED existenci proteinov\u00E9ho transport\u00E9ru v plazmatick\u00E9 membr\u00E1n\u011B, kter\u00FD umo\u017E\u0148uje transport netransferinov\u00E9ho \u017Eeleza do bu\u0148ky. Samotn\u00FD transport\u00E9r v\u0161ak nebyl zat\u00EDm identifikov\u00E1n. C\u00EDlem navrhovan\u00E9ho projektu je identifikace membr\u00E1nov\u00E9ho transport\u00E9ru netransferinov\u00E9ho \u017Eeleza. V p\u0159\u00EDpad\u011B nov\u00E9ho proteinu jej budeme detailn\u011Bji charakterizovat. Identifika\u010Dn\u00ED strategie je zalo\u017Eena na dvou p\u0159\u00EDstupech a jejich kombinaci: 1. \u017Eelezo v\u00E1zaj\u00EDc\u00ED proteiny ze solubilizovan\u00FDch membr\u00E1n budemedetekovat pomoc\u00ED afinitn\u00ED chromatografie na imobilizovan\u00FDch kovov\u00FDch iontech, 2. budeme konstruovat hybridomy produkuj\u00EDc\u00ED monoklon\u00E1ln\u00ED protil\u00E1tky, kter\u00E9 specificky inhibuj\u00ED transport netransferinov\u00E9ho \u017Eeleza. Protil\u00E1tky budou potom pou\u017Eity pro detekci a purifikaci transport\u00E9ru." . "Melanotransferin, integrin beta3 a SFT se ne\u00FA\u010Dastn\u00ED transportu netransferinov\u00E9ho \u017Eelena do bu\u0148ky. Transportu by se mohl \u00FA\u010Dastnit specifick\u00FD iontov\u00FD kan\u00E1l z\u00E1visl\u00FD na Ca2+, suspektn\u00ED annexin II a HSP 90,nov\u011B popsan\u00FD jako membr\u00E1nov\u00FD \u017Eelezo v\u00E1zaj\u00EDc\u00ED protein."@cs . "2007-01-15+01:00"^^ . "Cummulating data support the existence of plasma membrane protein transporter which facilitate non-transferrin iron transport into cells. However, the transporter itself has not been idenfified yet. The aim of proposed research is to identify membrane transporter of non-transferrin iron. In the case of a new protein we will characterize it in more detail. Identification strategy is based on two approaches and their combination: 1. iron-binding proteins of solubilized membranes will be detected by immobilized metal ion affinity chromatography, 2. hybridomas producing monoclonal antigodies, which specifically inhibit non-transferrin iron uptake, will be constructed. The antibodies will be employed to detect and purify the transporter."@en . . . "Membr\u00E1nov\u00FD transport netransferinov\u00E9ho \u017Eeleza u sav\u010D\u00EDch bun\u011Bk" . . . . " integrin beta3" . " melanotransferrin" . "IAA5052702" . . . "Membrane transport of non-transferrin iron in mammalian cells"@en . . " K562 cells" . . "membrane iron transport" . "1"^^ . "3"^^ . " HeLa cells" . " non-transferrin iron transport" . "0"^^ . "3"^^ . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=IAA5052702"^^ . . . " membrane iron-binding proteins" . . " SFT protein" . " HSP 90" . . "membrane iron transport; non-transferrin iron transport; membrane iron-binding proteins; HeLa cells, K562 cells; integrin beta3; SFT protein; melanotransferrin; HSP 90; annexin II;"@en .