. "8"^^ . "8"^^ . "0"^^ . . "Tato aplikace vyh\u00EDz\u00ED z na\u0161ich ned\u00E1vn\u00FDch n\u00E1lez\u016F, \u017Ee glykosyl-fofatidylinositol (GPI)- zakotven\u00E9 membr\u00E1nov\u00E9 glykoproteiny, CEA a Thy-1, v postnukle\u00E1rn\u00EDch supernatantech z bun\u011Bk COLO 205 a RBL, lyzovan\u00FDch v neiontov\u00FDch detergens, tvo\u0159\u00ED velk\u00E9 nekovalentn\u011B v\u00E1zan\u00E9 komplex s protein-tyrosin kin\u00E1zami (PTK) p56lek a p53/p56lyn, ale ne s pp60c-src. jA\u010Dkoliv GPI-zakotven\u00E9 glykoproteiny jsou slab\u011B rozpustn\u00E9 v neiontov\u00FDch detergens, nukle\u00E1rn\u00ED pelety, obsahuj\u00EDc\u00ED v\u011Bt\u0161inu t\u011Bchto protein\u016F, nebyly doposud studov\u00E1ny. Monoklon\u00E1ln\u00ED protil\u00E1tky specifick\u00E9 pro CEA, Thy-1, src-PTK a dal\u0161\u00ED budou vyu\u017Eity pro ur\u010Den\u00ED vnitrobun\u011B\u010Dn\u00E9 lokalizace c\u00EDlov\u00FDch struktur a zm\u011Bn v jejich rozpustnosti anebo aktivit\u011B u bun\u011Bk po stimulaci povrchov\u00FDch %22receptor\u016F%22. Studov\u00E1na bude rovn\u011B\u017E \u00FAloha cholesterolu v rozpustnosti GPI-komplex\u016F. Anal\u00FDzou p\u016Fsoben\u00ED frakcionovan\u00E9ho cytosolu na aktivaci permeabilizovan\u00FDch bun\u011Bk budou ur\u010Deny komponenty cytosolu, kter\u00E9 jsou nezbytn\u00E9 pro \u010Dasn\u00E1 stadia aktivace bun\u011Bk. Tato studia p\u0159isp\u011Bj\u00ED k pochopen\u00ED mechanismu" . . "1"^^ . . . "Function of GPI/kinase complexes"@en . . "IAA5052506" . . "0"^^ . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=IAA5052506"^^ . . "Funkce membr\u00E1nov\u00E9ho komplexu GPI/kin\u00E1za" . .