"1"^^ . " Src kinase" . . " Lyn kinase" . "0"^^ . . . " IgE receptor" . . . "Regula\u010Dn\u00ED funkce Lyn kin\u00E1zy p\u0159i aktivaci \u017E\u00EDrn\u00FDch bun\u011Bk" . "The first step in mast cell activation after aggregation of the high affinity IgE receptor (FceRI) is tyrosine phosphorylation of the receptor subunits. However, the regulation and consequences of Lyn kinase activity in resting and activated mast cells are not completely understood. In this proposal we will investigate how regulation of Lyn kinase activity may affect mast cell signaling through FceRI and GPI-anchored glycoproteins. Mutants of Lyn kinase defective in C-terminal tyrosine alone or in combination with mutations in catalytic, SH2 or SH3 domains will be prepared. Using a rapid Semliki Forest virus-based expression system, the mutants will be uxpressed in RBL-2H3 cells or bone marrow-derived mast cells from mice with defect in Lyn kinase and their properties will be analyzed using variety of techniques. Antibodies specific for C-terminal phosphotyrosine of Lyn will be prepared and used for analysis of distribution of hyperactive Lyn in the course of mast cell activation."@en . . . " Csk kinase" . . . . "IAA5052310" . "Regulatory roles of Lyn kinase in mast cell activation"@en . . "mast cell" . "Prvn\u00EDm krokem aktivace \u017E\u00EDrn\u00FDch bun\u011Bk po agragaci receptoru s vysokou afinitou pro IgE (FceRI) je fosforylace tyrosin\u016F receptorov\u00FDch subjednotek. Tato fosforylace je zah\u00E1jena Lyn kin\u00E1zou. Regulace aktivity Lyn kin\u00E1zy a jej\u00EDho p\u016Fsoben\u00ED na aktiva\u010Dn\u00ED d\u011Bje u klidov\u00FDch a aktivovan\u00FDch \u017E\u00EDrn\u00FDch bun\u011Bk v\u0161ak nen\u00ED kompletn\u011B zn\u00E1ma. V t\u00E9to grantov\u00E9 aplikaci chceme objasnit jak regulace aktivity Lyn kin\u00E1zy ovliv\u0148uje signalizaci prost\u0159ednictv\u00EDm FceRI a GPI zakotven\u00FDch protein\u016F. Budou p\u0159ipraveny mutanty Lyn kin\u00E1zy defektn\u00ED pouze v C-termin\u00E1ln\u00EDm tyrosinu nebo v kombinaci s defekty v katalytick\u00E9, SH2 nebo SH3 dom\u00E9n\u011B. Pomoc\u00ED syst\u00E9mu, kter\u00FD vyu\u017E\u00EDv\u00E1 modifikovan\u00E9ho Semliki Forest viru budou mutanty vpraveny do bun\u011Bk linie RBL-2H3 nebo do \u017E\u00EDrn\u00FDch z my\u0161\u00ED defektn\u00EDch v expresi Lyn kin\u00E1zy. Protil\u00E1tky proti C-termin\u00E1ln\u00EDmu fosfotyrosinu budou p\u0159ipraveny a vyu\u017Eity pro anal\u00FDzu distribuce (na \u00FArovni konfok\u00E1ln\u00EDho a elektronov\u00E9ho mikroskopu) a funkce hyperaktivn\u00EDho Lyn v pr\u016Fb\u011Bhu aktivace \u017E\u00EDrn\u00FDch bun\u011Bk." . "http://www.isvav.cz/projectDetail.do?rowId=IAA5052310"^^ . " Syk kinase" . . . " membrane microdomains" . . "Byly z\u00EDsk\u00E1ny nov\u00E9 v\u00FDsledky, kter\u00E9 ukazuj\u00ED, \u017Ee Lyn kin\u00E1za u FceRI-aktivovan\u00FDch \u017E\u00EDrn\u00FDch bun\u011Bk je regulovan\u00E1 \u0159adou faktor\u016F, jak\u00FDmi jsou adaptorov\u00FD protein NTAL, aktinov\u00FD cytoskelet a lipidick\u00E9 slo\u017Een\u00ED plasmatick\u00E9 membr\u00E1ny."@cs . " LAT adaptor" . "2009-04-03+02:00"^^ . "11"^^ . "11"^^ . "New data were obtained indicating that Lyn kinase in FceRI-activated mast cells is regulated by numerous factors, such as adaptor protein NTAL, actin cytoskeleton and lipidic composition of the plasma membrane"@en . . . "mast cell; IgE receptor; membrane microdomains; Lyn kinase; Src kinase; Csk kinase; Syk kinase; LAT adaptor; electron microscopy"@en . . . . . "0"^^ . .