. " Tribolium castaneum" . "2013-02-27+01:00"^^ . "Juveniln\u00ED hormon (JH) je posledn\u00EDm z hlavn\u00EDch lipofiln\u00EDch hormon\u016F, jeho\u017E skute\u010Dn\u00FD receptor nebyl zn\u00E1m. V tomto projektu byla definov\u00E1na JH-vazebn\u00E1 dom\u00E9na dosud jen p\u0159epokl\u00E1dan\u00E9ho receptoru Met, a byla prok\u00E1z\u00E1na funkce Met ve v\u00FDvoji a reprodukci hmyzu."@cs . " bHLH-PAS proteins" . . " Intracellular receptors" . . " Development" . "IAA500960906" . . "2009-01-01+01:00"^^ . "This project aims to clarify the enigma of juvenile hormone (JH) action. JH regulates insect development by preventing metamorphosis in larvae that have not yet reached the appropriate stage. While four decades have passed since the sesquiterpenoid JH structure was discovered, we still do not know how this hormone works, what receptor and signaling pathway it uses. Such knowledge is important because insects are the most numerous of animals on the Earth, and metamorphosis contributed to their success. Perturbing of JH signaling is a good way to control insect pests. The exquisite model beetle Tribolium has enabled us to show that JH regulates metamorphosis through a Methoprene-tolerant (Met) protein of the bHLH-PAS family. Here we propose to determine how the transcription factor Met transduces the JH signal and whether it serves as a JH receptor. We plan to disclose the molecular structure and interactions of Met, its binding and activation by JH, and to identify its target genes."@en . . "Juvenile hormone; Intracellular receptors; bHLH-PAS proteins; Development; Insect metamorphosis; Tribolium castaneum; Drosophila melanogaster"@en . . . "1"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=IAA500960906"^^ . . "0"^^ . "Juvenile hormone (JH) was the last major lipophilic hormone without a bone fide receptor. In this project, a JH-binding domain of the candidate receptor Met has been defined, and the function of Met in insect development and reproduction demonstrated."@en . "2014-04-14+02:00"^^ . . . " Insect metamorphosis" . . . "0"^^ . . . "5"^^ . "5"^^ . . "C\u00EDlem projektu je objasnit z\u00E1hadu p\u016Fsoben\u00ED juveniln\u00EDho hormonu (JH). JH \u0159\u00EDd\u00ED v\u00FDvoj hmyzu t\u00EDm, \u017Ee br\u00E1n\u00ED larv\u00E1m vstoupit do metamorfosy d\u0159\u00EDve, ne\u017E dos\u00E1hnou pot\u0159ebn\u00E9ho stadia. Od objevu seskviterpenoidn\u00ED struktury JH ub\u011Bhla \u010Dty\u0159i desetilet\u00ED, av\u0161ak dosud nen\u00ED zn\u00E1mo jak tento hormon funguje, p\u0159es jak\u00FD receptor a sign\u00E1ln\u00ED dr\u00E1hu p\u016Fsob\u00ED. Takov\u00E9 znalosti jsou d\u016Fle\u017Eit\u00E9, jeliko\u017E hmyz je nejpo\u010Detn\u011Bj\u0161\u00ED skupinou \u017Eivo\u010Dich\u016F na Zemi a metamorfosa k jeho \u00FAsp\u011B\u0161nosti p\u0159isp\u011Bla. Naru\u0161en\u00ED signalizace JH je vhodnou cestou kontroly hmyz\u00EDch \u0161k\u016Fdc\u016F. V\u00FDhodn\u00E9 vlastnosti nov\u00E9ho modelu, brouka Tribolium, n\u00E1m umo\u017Enily prok\u00E1zat, \u017Ee JH reguluje metamorfosu prost\u0159ednictv\u00EDm proteinu Methoprene-tolerant (Met) z rodiny bHLH-PAS. Nyn\u00ED navrhujeme zjistit, jak\u00FDm zp\u016Fsobem transkrip\u010Dn\u00ED faktor Met p\u0159en\u00E1\u0161\u00ED sign\u00E1l JH a zda s\u00E1m slou\u017E\u00ED jako vnitrobun\u011B\u010Dn\u00FD receptor JH. Pl\u00E1nujeme odhalit molekul\u00E1rn\u00ED strukturu a interakce proteinu Met, prok\u00E1zat jeho aktivaci vazbou na JH, a naj\u00EDt jeho c\u00EDlov\u00E9 geny." . "Juveniln\u00ED hormon: mechanismus p\u016Fsoben\u00ED b\u011Bhem metamorfosy hmyzu" . "Juvenile hormone action during insect metamorphosis"@en . . "Juvenile hormone" . . "2013-12-31+01:00"^^ .