. "Development of a Novel Recombinogenic Technique for Chloroplast Transformation and its Use for Production of human papillomavirus E7 Protein in Plants"@en . "Je navr\u017Een v\u00FDvoj nov\u00E9 metody transformace chloroplast\u016F vyu\u017E\u00EDvaj\u00EDc\u00ED line\u00E1rn\u00ED DNA vektory aplikovan\u00E9 biolisticky spolu s chloroplastov\u00FDmi kazetami exprimuj\u00EDc\u00EDmi rekombina\u010Dn\u00ED geny Red bakteriof\u00E1ga lambda. Idea vych\u00E1z\u00ED z pokus\u016F o c\u00EDlenou inser\u010Dn\u00ED mutagenezi v kvasink\u00E1ch, sinic\u00EDch a E. coli, kde konce DNA podporuj\u00ED v\u00FDrazn\u011B homologn\u00ED rekombinaci a lambdov\u00E9 proteiny Exo, Beta a Gam velmi \u00FA\u010Dinn\u011B chr\u00E1n\u00ED a upravuj\u00ED konce DNA, kter\u00E9 pak i p\u0159i velmi kr\u00E1tk\u00E9 homologii v\u00FDrazn\u011B podporuj\u00ED rekombinaci. Line\u00E1rn\u00ED vektory mohou b\u00FDt p\u0159ipraveny jako produkty PCR reakce se syntetick\u00FDmi primery na konc\u00EDch, homologn\u00EDmi s c\u00EDlovou sekvenc\u00ED v chloroplastov\u00E9m genomu. Chloroplastov\u00E9 vektory ponesou krom\u011B markerov\u00FDch gen\u016F tak\u00E9 onkogen E7 z lidsk\u00E9ho papilomaviru ve form\u011B neonkogenn\u00ED, ale vysoce immunogenn\u00ED fuze E7GGG/GUS. Cilem je z\u00EDskat transformanty produkuj\u00EDc\u00ED antigen vyu\u017Eiteln\u00FD jako sou\u010D\u00E1st terapeutick\u00E9 vakciny. Virov\u00E9 antigeny produkovan\u00E9 v rostlin\u00E1ch budou stanovov\u00E1ny protil\u00E1tkami a testov\u00E1ny na my\u0161\u00EDch." . "transgenic plants; chloroplast transformation; lambda Red recombination; human papilloma virus; cervical carcinoma"@en . "http://www.isvav.cz/projectDetail.do?rowId=IAA500960903"^^ . . . " human papilloma virus" . . . . "Tobacco and Chlamydomonas strains were prepared with chloroplasts transformed by semisynt. vectors carrying human papilloma virus E7 oncogen. Algal extracts were used for mice immunization where E7-antibodies were induced but not cytotoxic lymphocytes."@en . "2012-12-31+01:00"^^ . "Our aim is to develop more efficient method for plant chloroplast transformation using biolistic bombardment with linear DNA vectors together with chloroplast cassettes expressing bacteriophage lambda Red proteins. The idea stems from experiments with targeted insertion mutagenesis in yeast, cyanobacteria, and E. coli, where DNA ends promote recombination, and lambda Red proteins Exo, Beta, and Gam are very efficient in short recombinogenic ends protection and processing. Linear vectors can be generated conveniently as PCR products with synthetic primer ends that are homologous to sequence targets in the chloroplast genome. Chloroplast vectors will carry marker genes and also human papillomavirus oncogene E7 in the form of highly immunogenic but nononcogenic fusion E7GGG/GUS. It is our goal to obtain transplastomic plants producing this antigen with potential use as therapeutic vaccine. Viral antigens produced in plants will be assayed with antibodies and tested in mice."@en . . "2012-02-29+01:00"^^ . . "1"^^ . . "0"^^ . . "1"^^ . . "Byly p\u0159ipraveny linie tab\u00E1ku a Chlamydomonas s chloroplasty transformovan\u00FDmi polosyntetick\u00FDmi vektory, nesouc\u00EDmi onkogen E7 z lidsk\u00E9ho papillomaviru. Extrakty z \u0159asy byly testov\u00E1ny na my\u0161\u00EDch, kde vyvolaly protil\u00E1tkovou, ale nikoli cytotoxickou odpov\u011B\u010F."@cs . "2"^^ . " chloroplast transformation" . . " lambda Red recombination" . "1"^^ . "IAA500960903" . . . "2014-02-12+01:00"^^ . "transgenic plants" . . "V\u00FDvoj nov\u00E9 rekombina\u010Dn\u00ED technologie k transformaci chloroplast\u016F a jej\u00ED vyu\u017Eit\u00ED pro produkci E7 proteinu lidsk\u00E9ho papilomaviru v rostlin\u00E1ch" . . . . . "2009-01-01+01:00"^^ .