"1"^^ . "Anal\u00FDza mechanizm\u016F autokrinn\u00EDch \u00FA\u010Dink\u016F adipokin\u016F na metabolizmus gluk\u00F3zy a lipid\u016F" . . . "IAA500110805" . . "The role of endocrine function of adipose tissue in the pathogenesis of insulin resistance and dyslipidemia has begun to attract increasing attention, however, the underlying mechanisms are not fully understood. Even less is known about in vivo autocrine effects of adipokines on adipose tissue glucose and lipid metabolism due to lack of appropriate animal models. In the current proposal, we will test a hypothesis that autocrine effects of adipokines play important role in the adipose tissue sensitivity to insulin action and subsequently in the pathogenesis of insulin resistance. Specifically, we will use the RNA interference in 3T3-L1 adipocytes to analyze metabolic effects associated with down regulation of selected adipokines (visfatin, resistin, leptin and adiponectin). The underlying mechanisms will be studied by analyzing the expression of genes involved in the pathogenesis of obesity and insulin resistance, including genes coding receptors, transporters, nuclear receptors, etc."@en . . "autocrine effects; adipokine; metabolic syndrome"@en . . "Analysis of mechanisms of adipokine autocrine effects on glucose and lipid metabolism"@en . . . . "12"^^ . "2011-12-31+01:00"^^ . . "autocrine effects" . "12"^^ . " adipokine" . . . . . "4"^^ . "http://www.isvav.cz/projectDetail.do?rowId=IAA500110805"^^ . "0"^^ . . "2013-06-28+02:00"^^ . "2011-03-18+01:00"^^ . "CobB1 sirtuin Streptomyces coelicolor deacetylate AcetylCoAsynthetase(acsA. Acetylated acsA loses the ability to transfer acetate to CoA.Deacetylation of the enzyme required CobB1,NAD,ATP-Mg.Two new inhibitors ofCobB1OH-deriv. of antraquinone were found."@en . "V patogenezi inzulinov\u00E9 rezistence a dyslipid\u00E9mie se zd\u00E1 b\u00FDt st\u00E1le v\u00FDznamn\u011Bj\u0161\u00ED \u00FAloha endokrinn\u00ED funkce tukov\u00E9 tk\u00E1n\u011B, av\u0161ak odpov\u011Bdn\u00E9 mechanizmy nejsou zcela zn\u00E1my. Je\u0161t\u011B m\u00E9n\u011B se v\u00ED o in vivo autokrinn\u00EDch \u00FA\u010Dinc\u00EDch adipokin\u016F na metabolizmus gluk\u00F3zy a lipid\u016F v tukov\u00E9 tk\u00E1ni, p\u0159edev\u0161\u00EDm kv\u016Fli nedostatku vhodn\u00FDch zv\u00ED\u0159ec\u00EDch model\u016F. V p\u0159edkl\u00E1dan\u00E9m projektu budeme testovat hypot\u00E9zu, \u017Ee autokrinn\u00ED \u00FA\u010Dinky adipokin\u016F hraj\u00ED d\u016Fle\u017Eitou \u00FAlohu v regulaci citlivosti tukov\u00E9 tk\u00E1n\u011B k \u00FA\u010Dink\u016Fm inzulinu a n\u00E1sledn\u011B v patogenezi inzulinov\u00E9 rezistence. Konkr\u00E9tn\u011B budeme pomoc\u00ED RNA interference u 3T3-L1 adipocyt\u016F studovat \u00FA\u010Dinky sn\u00ED\u017Een\u00E9 exprese vybran\u00FDch adipokin\u016F (visfatin, resistin, leptin, adiponektin) na metabolick\u00E9 parametry. Odpov\u011Bdn\u00E9 mechanizmy budou analyzov\u00E1ny pomoc\u00ED studia exprese gen\u016F d\u016Fle\u017Eit\u00FDch v patogenezi obezity a inzulinov\u00E9 rezistance, v\u010Detn\u011B gen\u016F k\u00F3duj\u00EDc\u00EDch receptory, transport\u00E9ry, jadern\u00E9 receptory, metabolick\u00E9 enzymy, sekretovan\u00E9 faktory, proteiny sign\u00E1ln\u00ED transdukce a transkrip\u010Dn\u00ED faktory." . "2008-01-01+01:00"^^ . . . . "CobB1 sirtuin katalyzuje deacetylaci acetylCoA synthetasy u Streptomyces coelicolor.Acetylovan\u00FD enzym ztr\u00E1c\u00ED aktivitu p\u0159i p\u0159enosu acet\u00E1tu na CoA. Deacetylace je z\u00E1visl\u00E1 na NAD,CobB1,ATP-Mg.Byly nalezeny dva nov\u00E9 inhibitory CobB1.OH-deriv\u00E1ty antrachinonu."@cs . .