"P\u0159ena\u0161e\u010De typu Na+/H+ jsou p\u0159\u00EDtomny ve v\u0161ech organismech a pod\u00EDlej\u00ED se na udr\u017Eov\u00E1n\u00ED homeost\u00E1ze kationt\u016F alkalick\u00FDch kov\u016F a vnitrobun\u011B\u010Dn\u00E9ho pH. V modelov\u00E9 kvasince Saccharomyces cerevisiae existuj\u00ED t\u0159i z\u00E1stupci rodiny Na/H antiporter\u016F - Nha1p v plasmatick\u00E9 membr\u00E1n\u011B, Kha1p v membr\u00E1n\u011B Golgiho apar\u00E1tu a Nhx1p v membr\u00E1n\u00E1ch endosom\u00E1ln\u00EDch v\u00E1\u010Dk\u016F a prevakuol\u00E1rn\u00EDch kompartment\u016F. A\u010Dkoli substr\u00E1tov\u00E1 specifita, bun\u011B\u010Dn\u00E1 lokalizace a z\u00E1kladn\u00ED \u00FAloha t\u011Bchto t\u0159\u00ED p\u0159ena\u0161e\u010D\u016F ji\u017E byly objasn\u011Bny, st\u00E1le je\u0161t\u011B nejsou zn\u00E1my v\u0161echny jejich biologick\u00E9 funkce. P\u0159edkl\u00E1dan\u00FD projekt m\u00E1 za c\u00EDl podrobnou charakterizaci 1) fyziologick\u00FDch \u00FAloh , 2) biogeneze a degradace t\u011Bchto transport\u00E9r\u016F. Budou p\u0159ipraveny mutantn\u00ED kmeny postr\u00E1daj\u00EDc\u00ED/exprimuj\u00EDc\u00ED v r\u016Fzn\u00FDch kombinac\u00EDch geny pro jednotliv\u00E9 p\u0159ena\u0161e\u010De a geny pod\u00EDlej\u00EDc\u00ED se na tvorb\u011B membr\u00E1n a bun\u011B\u010Dn\u00FDch organel \u010Di biogenezi a degradaci membr\u00E1nov\u00FDch protein\u016F. Mikrobiologick\u00FDmi, biochemick\u00FDmi, biofyzik\u00E1ln\u00EDmi a imunochemick\u00FDmi metodami budou tyto kmeny studov\u00E1ny." . . " intracellular pH" . . . "2012-12-31+01:00"^^ . " potassium homeostasis" . . . "0"^^ . . "1"^^ . . "Na+/H+ antiporters serve in all organisms mainly to maintain intracellular homeostasis of alkali metal cations and pH. In the model yeast Saccharomyces cerevisiae, three members of the family exist: Nha1p is in the plasma membrane, Kha1 in the Golgi apparatus and the Nhx1 antiporter is localized in endosomal/prevacuolar membranes. Though the substrate specificity, cell localization and main role of these three antiporters have been described, a detailed characterization of all their biological functions has not been performed. This project aims to characterize their physiological functions, biogenesis and degradation. Mutant strains lacking/overexpressing genes for transporters in combination with genes involved in membrane and organelle synthesis, biogenesis or degradation of membrane proteins will be prepared and studied using microbiological, biochemical, biophysical and immunochemical methods."@en . . "Na+/H+ antiporters" . "0"^^ . . . "16"^^ . "16"^^ . . "2008-01-01+01:00"^^ . . "\u00DAloha p\u0159ena\u0161e\u010D\u016F Na/H v bun\u011B\u010Dn\u00E9 fyziologii - souhra transport\u00E9r\u016F v optimalizaci vnitrobun\u011B\u010Dn\u00E9 homeost\u00E1ze K+ a pH" . . . "2014-02-12+01:00"^^ . "Role of Nha1 antiporters in the regulation of intracellular cation content, membrane potential and intracellular pH in yeast has been described. Four genes involved in the osmotolerance and homeostasis via interaction with Nha1 have been identified."@en . "Role of Na/H antiporters in cell physiology - transporters teamwork in intracellular pH and K+ homeostasis"@en . . . . . "http://www.isvav.cz/projectDetail.do?rowId=IAA500110801"^^ . "2012-02-29+01:00"^^ . . . "Na+/H+ antiporters; potassium homeostasis; intracellular pH; yeast"@en . "IAA500110801" . "Byla pops\u00E1na role transport\u00E9r\u016F typu Nha1 v optimalizaci vnitrobun\u011B\u010Dn\u00E9 koncentrace kationt\u016F alkalick\u00FDch kov\u016F, membr\u00E1nov\u00E9ho potenci\u00E1lu a vnitrobun\u011B\u010Dn\u00E9ho pH kvasinek. Byly nalezeny 4 dal\u0161\u00ED geny pod\u00EDlej\u00EDc\u00ED se na osmotoleranci a homeost\u00E1zi interakc\u00ED s Nha1."@cs .