"DNA damage" . "Interactions of wild type and mutant p53 proteins with damaged DNA and their roles in cellular response to anticancer chemotherapy"@en . "2010-03-09+01:00"^^ . . " molecular recognition" . . "Interakce standardn\u00EDho a mutantn\u00EDch protein\u016F p53 s po\u0161kozenou DNA a jejich v\u00FDznam p\u0159i odpov\u011Bdi bun\u011Bk na protin\u00E1dorovou chemoterapii" . . . "2013-06-28+02:00"^^ . " mutant p53" . . " genotoxic substances" . . . . . . "Byly prozkoum\u00E1ny sekven\u010Dn\u011B specifick\u00E9 a strukturn\u011B strukturn\u011B selektivn\u00ED interakce protein\u016F p53 a p73, v\u010Detn\u011B jejich schopnosti rozpozn\u00E1vat chemicky modifikovanou DNA. Byly zavedeny nov\u00E9 experiment\u00E1ln\u00ED techniky pro studium DNA-protein interakc\u00ED."@cs . " DNA-protein interactions" . "Interakce n\u00E1dorov\u011B supresorov\u00E9ho proteinu p53 s DNA maj\u00ED kl\u00ED\u010Dov\u00FD v\u00FDznam pro jeho biologick\u00E9 aktivity. Bylo navr\u017Eeno, \u017Ee nejen sekven\u010Dn\u011B-specifick\u00E1 vazba standardn\u00EDho (wt) p53, ale t\u00E9\u017E schopnost wt i mutantn\u00EDch (mut) p53 rozpozn\u00E1vat strukturu DNA m\u00E1 v\u00FDznam pro jejich charakteristick\u00E9 funkce. V tomto projektu navrhujeme zkoumat interakce wt a mut p53 s po\u0161kozenou DNA, zejm\u00E9na s DNA modifikovanou protirakovinn\u00FDmi l\u00E9\u010Divy. Tyto studie budou zam\u011B\u0159eny na (1) schopnost mut p53 rozpoznat specifick\u00E1 po\u0161kozen\u00ED DNA, (2) vliv po\u0161kozen\u00ED DNA na sekven\u010Dn\u011B-specifickou vazbu wt p53, a (3) vliv genotoxick\u00FDch l\u00E1tek na interakce mut p53 se specifick\u00FDmi \u00FAseky genomov\u00E9 DNA, kter\u00E9 byly identifikov\u00E1ny jako p\u0159irozen\u00E1 vazebn\u00E1 m\u00EDsta mut p53 in vivo. V prvn\u00ED f\u00E1zi budou biochemick\u00FDmi a molekul\u00E1rn\u011B biologick\u00FDmi metodami zkoum\u00E1ny p\u0159edev\u0161\u00EDm interakce purifikovan\u00FDch protein\u016F s DNA v bezbun\u011B\u010Dn\u00E9m syst\u00E9mu, pot\u00E9 rovn\u011B\u017E vliv genotoxick\u00FDch l\u00E1tek na interakce wt a mut p53 s DNA na \u00FArovni modelov\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED." . "http://www.isvav.cz/projectDetail.do?rowId=IAA500040701"^^ . " tumor suppressor p53" . "2010-12-31+01:00"^^ . . "Sequence-specific and structure-selective DNA interactions of p53 and p73 proteins were described, including their ability to recognize chemically modified DNA. New experimental techniques for DNA-protein interaction studies were introduced."@en . "DNA damage; tumor suppressor p53; mutant p53; DNA-protein interactions; molecular recognition; DNA conformation; cancer; genotoxic substances; anticancer drugs"@en . . "2007-01-01+01:00"^^ . . . . "7"^^ . . "7"^^ . "IAA500040701" . " DNA conformation" . "0"^^ . . "2"^^ . " cancer" . . "Interactions of tumor suppressor protein p53 with DNA are crucial for its biological activities. It was suggested that not only the wild type (wt) p53 sequence-specific DNA binding, but also ability of both wt and mutant (mut) p53 to recognize DNA structure, are important for their characteristic functions. We propose to study wt and mut p53 interactions with damaged DNA, particularly DNA treated with antitumor drugs. These studies will be focused on (1) the ability of mutp53 to recognize specific DNA damage, (2) effects of DNA damage on the wt p53 sequence-specific DNA binding, and (3) influence of genotoxic substances on mut p53 binding to genomic DNA elements identified as its natural binding sites in vivo. Using biochemical and molecular-biological techniques, interactions of purified proteins with DNA in cell-free system will be investigated in the first stage, followed by studies of the influence of genotoxic agents on p53-DNA binding at the level of model cell lines."@en . "1"^^ . . .