"17"^^ . " brassinosteroid" . "IAA400550801" . "2012-12-31+01:00"^^ . "Synthesis of tritium labelled brassinosteroids and their application in the studies of their mechanism of action on molecular level."@en . "Byly vypracov\u00E1ny postupy pro p\u0159\u00EDpravu brassinosteroid\u016F zna\u010Den\u00FDch tritiem. T\u0159i tritiem zna\u010Den\u00E9 brassinosteroidy byly pou\u017Eity k testov\u00E1n\u00ED n\u00E1vratnosti imunoafinitn\u00EDch chromatografick\u00FDch kolon a k vazebn\u00FDm studi\u00EDm na estrogenn\u00EDch receptorech."@cs . . " anticancer activity" . . . " reduction of 1" . . . "The anticancer activities of brassinosteroids \u2013 plant growth hormones \u2013 on human cells have been recently recognised. To study the mechanism of action of these compounds on molecular level their labelling with tritium at specific activity higher than 60 Ci.mmol-1 is indispensable. Proposed labelling method is based on partial oxidation of vicinal hydroxy groups to hydroxy ketones and their reaction with carbonyl compound to form a 1,3-dioxolene derivative. Catalytic reduction of 1,3-dioxolene ring with carrier free tritium followed by hydrolysis will give the starting compound tritiated in stable positions. Radioligand binding assays with 24-[3H]epibrassinolide and 28 [3H]homocastasterone on human estrogene Er-alfa and Er-beta receptors could let to explanation if the observed anticancer activity is related to brassinosteroid-nuclear receptor interactions. The tritium labelled brassinosteroids will be also used for determination of recovery during their isolation from plant materials."@en . . . "2014-02-12+01:00"^^ . . "The synthetic pathways for labeling of brassinosteroids by tritium were developed. The tritiated brassionsteroids were used for testing of brassinosteroids recovery from immunoaffinity chromatographic columns and for binding assays on estrogen receptors."@en . "3-dioxolene ring" . "Brassinosteroidy jsou r\u016Fstov\u00E9 hormony rostlin. Krom\u011B jejich studia z hlediska vyu\u017Eit\u00ED v zem\u011Bd\u011Blstv\u00ED byl z\u00E1jem o tyto slou\u010Deniny umocn\u011Bn objevem jejich protin\u00E1dorov\u00E9 aktivity na lidsk\u00E9 n\u00E1dorov\u00E9 bu\u0148ky. Studium mechanismu \u00FA\u010Dinku brassinosteroid\u016F na molekul\u00E1rn\u00ED \u00FArovni vy\u017Eaduje jejich zna\u010Den\u00ED tritiem. Pro zaveden\u00ED \u010Dty\u0159 atom\u016F tritia (c\u00EDlem je specifick\u00E1 aktivita vy\u0161\u0161\u00ED ne\u017E 60 Ci.mmol-1) do molekuly bude vyu\u017Eito dvou vicin\u00E1ln\u00EDch diolov\u00FDch skupin, typick\u00FDch pro v\u0161echny brassinosteroidy. Jejich parci\u00E1ln\u00ED oxidac\u00ED budou p\u0159ipraveny vicin\u00E1ln\u00ED hydroxyketony, kter\u00E9 reakc\u00ED s karbonylovou slou\u010Deninou poskytnou 1,3-dioxolenov\u00FD deriv\u00E1t. Katalytick\u00E1 tritiace 1,3-dioxolenov\u00E9ho deriv\u00E1tu beznosi\u010Dov\u00FDm tritiov\u00FDm plynem a n\u00E1sledn\u00E1 hydrol\u00FDza poskytnou v\u00FDchoz\u00ED biologicky aktivn\u00ED slou\u010Deninu zna\u010Denou tritiem ve stabiln\u00EDch poloh\u00E1ch. Studium vazby 24-[3H]epibrassinolidu a 28-[3H]homocastasteronu na lidsk\u00E9 estrogenov\u00E9 Er-alfa a Er-beta receptory by m\u011Blo objasnit jejich protin\u00E1dorovou aktivitu na molekul\u00E1rn\u00ED \u00FArovni." . . . . . "2008-01-01+01:00"^^ . . "1"^^ . "2"^^ . "http://www.isvav.cz/projectDetail.do?rowId=IAA400550801"^^ . "0"^^ . " estrogen receptors" . . . "tritium; brassinosteroid; reduction of 1,3-dioxolene ring; estrogen receptors; anticancer activity; immunoaffinity purification"@en . . "2012-02-29+01:00"^^ . . "tritium" . . . "P\u0159\u00EDprava tritiem zna\u010Den\u00FDch brassinosteroid\u016F a jejich aplikace p\u0159i studiu mechanismu \u00FA\u010Dinku brassinosteroid\u016F na molekul\u00E1rn\u00ED \u00FArovni." . . "17"^^ .