"IAA4004801" . "0"^^ . . . "0"^^ . "4"^^ . . "4"^^ . . "1"^^ . . . . . . . "V\u011Bt\u0161ina chemick\u00FDch nukle\u00E1z je zalo\u017Eena na redoxn\u00EDch reakc\u00EDch iont\u016F p\u0159echodn\u00FDch kov\u016F, p\u0159i nich\u017E vznikaj\u00ED voln\u00E9 radik\u00E1ly atakuj\u00EDc\u00ED zbytky deoxyrib\u00F3zy a b\u00E1z\u00ED v molekul\u00E1ch DNA, co\u017E vede p\u0159\u00EDmo \u010Di nep\u0159\u00EDmo k jejich \u0161t\u011Bpen\u00ED. Tyto reakce obvykle vy\u017Eaduj\u00ED p\u0159\u00EDtomnost reduk\u010Dn\u00EDho \u010Dinidla. Navrhujeme vyu\u017E\u00EDt mo\u017Enosti citliv\u00E9 kontroly elektrochemick\u00E9ho potenci\u00E1lu elektrod pro regulaci \u0161t\u011Bpen\u00ED DNA imobilizovan\u00E9 na povrchu rtu\u0165ov\u00FDch (HMDE), rtu\u0165ov\u00FDch filmov\u00FDch (MFE) a uhl\u00EDkov\u00FDch (CE) elektrod chemick\u00FDmi nukle\u00E1zami v nep\u0159\u00EDtomnosti chemick\u00FDch reduk\u010Dn\u00EDch \u010Dinidel. Bude u\u010Din\u011Bn pokus odli\u0161it p\u0159\u00EDmou tvorbu zlom\u016F v \u0159et\u011Bzc\u00EDch DNA od tvorby m\u00EDst labiln\u00EDch v\u016F\u010Di alk\u00E1li\u00EDm. Pro detekci \u0161t\u011Bpen\u00ED a po\u0161kozen\u00ED DNA budou vyu\u017Eity jednak sign\u00E1ly specifick\u00E9 pro zlomy v ret\u011Bzc\u00EDch DNA (zejm\u00E9na na HMDE, p\u0159\u00EDpadn\u011B MFE), jednak zm\u011Bny sign\u00E1l\u016F zbytk\u016F jednotliv\u00FDch elektroaktivn\u00EDch b\u00E1z\u00ED (guaninu, adeninu a cytosinu na HMDE, p\u0159\u00EDp. MFE, a guaninu a adeninu na CE)." . "Electrodes as regulators of the clea vage of the immobilized DNA by redox-modulated chemical nucleases"@en . "http://www.isvav.cz/projectDetail.do?rowId=IAA4004801"^^ . . "\u0160t\u011Bpen\u00ED DNA na rtu\u0165ov\u00E9 elektrod\u011B v p\u0159\u00EDt. kysl\u00EDku a p\u0159echodn\u00FDch kov\u016F je modulov\u00E1no potenci\u00E1lem elektrody. Rtu\u0165ov\u00E1 filmov\u00E1 elektroda m\u016F\u017Ee slou\u017Eit jako senzor pro po\u0161kozen\u00ED DNA. Komplexy DNA s interkal\u00E1tory vykazuj\u00ED specifick\u00E9 voltametrick\u00E9 chov\u00E1n\u00ED."@cs . "The principle of supercoiled DNA immobilized at the electrode surface will be applied in the studies of DNA cleavage and damage by chemical nucleases. Most of chemical nucleases involve redox reactions of transition metal ions in which free radicals are generated; the latter attack deoxyribose and/or base residues in the DNA molecules, thus directly or indirectly cleaving DNA strands. The above reactions usually require a reducing agent. We propose to utilize the possibility of sensitive electrode potential control for the regulation of the cleavage of DNA immobilized at the surface of mercury (HMDE), mercury film (MFE) and carbon (CE) electrodes by chemical reductants. For the detection of the DNA cleavage and damage either the signals specific for DNA strand breaks (at HMDE or MFE) or the changes of intrinsic redox signals of base residues (guanine, adenine and cytosine at HMDE or MFE, and guanine and adenine at CE) will be used."@en . . "Elektrody jako regul\u00E1tory \u0161t\u011Bpen\u00ED imobilizovan\u00E9 DNA chemick\u00FDmi nukle\u00E1zami z\u00E1visl\u00FDmi na redoxn\u00EDch reakc\u00EDch" . . . . .