"P\u0159enos nabit\u00FDch a nenabit\u00FDch \u010D\u00E1stic p\u0159es modelov\u00E9 biologick\u00E9 membr\u00E1ny" . "Phospholipids; Phospholipids bilayer; Model cell membrane; Ionic channels; Cell permeable peptides; Electrochemical impedance spectroscopy; Electrochemistry"@en . "http://www.isvav.cz/projectDetail.do?rowId=IAA400400806"^^ . . "Realizovan\u00E9 experimenty umo\u017Enily charakterizovat transport n\u011Bkter\u00FDch t\u011B\u017Ek\u00FDch kov\u016F(Cd,Cu)ve form\u011B voln\u00FDch iont\u016F \u010Di komplex\u016F s n\u00EDzkomolekul\u00E1rn\u00EDmi org.kyselinami p\u0159es modelov\u00E9 fosfolipidov\u00E9 membr\u00E1ny,stabilizovan\u00E9 na por\u00E9zn\u00EDm,amalg\u00E1mov\u00E9m \u010Di agarov\u00E9m nosi\u010Di."@cs . "2014-02-12+01:00"^^ . . . . . "2008-01-01+01:00"^^ . "The transporting processes of some heavy metals (e.g., Cu, Cd)(in the form of free ions or complexes with low molecular weight organic acids)across model supported phospholipid membranes in porous, at amalgam or at agar substrates were characterized."@en . "0"^^ . . "0"^^ . " Electrochemical impedance spectroscopy" . . "1"^^ . . " Cell permeable peptides" . . . . " Ionic channels" . . "Phospholipids" . . "Transport of Charged and Uncharged Species across Models of Biological Membranes"@en . . . "95"^^ . "C\u00EDlem projektu je p\u0159\u00EDprava a charakterizace fosfolipidov\u00FDch dvojvrstev na stabilizovan\u00FDch f\u00E1zov\u00FDch rozhran\u00EDch,kter\u00E1 umo\u017E\u0148uj\u00ED studium p\u0159enosu nabit\u00FDch \u010D\u00E1stic z jedn\u00E9 f\u00E1ze do druh\u00E9.P\u0159enos anorganick\u00FDch iont\u016F lze nejl\u00E9pe studovat s pou\u017Eit\u00EDm rtuti,jako jedn\u00E9 z f\u00E1z\u00ED, vzhledem k rozs\u00E1hl\u00E9mu objemu relevantn\u00EDch elektrochemick\u00FDch dat,zat\u00EDmco p\u0159enos slo\u017Eit\u011Bj\u0161\u00EDch molekul bude studov\u00E1n s pou\u017Eit\u00EDm vodn\u00E9 f\u00E1ze zpevn\u011Bn\u00E9 agarov\u00FDm gelem. Studium p\u0159enosu iont\u016F a molekul p\u0159es takov\u00E1to rozhran\u00ED p\u0159isp\u011Bje k v\u00FDzkumu proces\u016F na biologick\u00FDch membr\u00E1n\u00E1ch.Za t\u00EDmto \u00FA\u010Delem budou do p\u0159ipraven\u00FDch modelov\u00FDch biologick\u00FDch membr\u00E1n inkorporov\u00E1ny peptidy a polypeptidy, kter\u00E9 jsou schopny vytv\u00E1\u0159et iontov\u00E9 kan\u00E1lky a p\u0159en\u00E1\u0161et bu\u010F jednotliv\u00E9 ionty nebo molekuly p\u0159es fosfolipidov\u00E9 membr\u00E1ny.Mo\u017Enosti transportu zvolen\u00FDch l\u00E1tek p\u0159es model. membr\u00E1ny bude zkoum\u00E1na t\u00E9\u017E pomoc\u00ED penetra\u010Dn\u00EDch peptid\u016F. Navr\u017Een\u00FD v\u00FDzkum zahrnuje p\u0159edev\u0161\u00EDm elektrochemick\u00E9 a elektroanalytick\u00E9 metody (cyklick\u00E1 voltam.,impedan\u010Dn\u00ED spektroskopie,konduktometrie)." . . "95"^^ . " Phospholipids bilayer" . . . " Model cell membrane" . "2012-02-29+01:00"^^ . "IAA400400806" . "2012-12-31+01:00"^^ . "The project is aimed at the preparation and characterization of phospholipid bilayers at stabilized phase boundaries enabling studies of transfer of charged and uncharged species between the two phases.The best way for investigation of inorganic ion transfer is provided by using mercury as one of the phases,in view of the huge volume of the relevant electrochemical data available,whereas the transfer of more complex species is better studied in the system of an organic and an aqueous phase stabilized by agar gel.The results contribute to the understanding of ion transport across real membranes.Polypeptides and peptides capable forming ionic channels and enabling transport of various ions and molecules across the membranes,will be incorporated into the model membranes.The possibilities of transport of selected compounds across the model membrane will be investigated using cell permeable peptides as well.The investigation is primarily based on electrochem.methods(e.g.,CV,IS,conductom.)."@en . . .