"2008-02-21+01:00"^^ . . "44"^^ . "DNA determination; DNA hybridization; purine derivatives; voltammetry; chronopotentiometry"@en . "44"^^ . . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=IAA100040602"^^ . . . . "1"^^ . "New approaches in electrochemical analysis of nucleic acids and oligonucleotides aimed at a ultrasensitive microdetection of DNA and DNA hybridization."@en . " DNA hybridization" . . "DNA determination" . . . "0"^^ . . . "2"^^ . . . . . "Projekt se zab\u00FDval anal\u00FDzou oligonukleotid\u016F a jejich slo\u017Eek pomoc\u00ED adsorptivn\u00ED rozpou\u0161t\u011Bc\u00ED voltametrie v kombinaci s elimina\u010Dn\u00ED voltametri\u00ED s c\u00EDlem z\u00EDskat nov\u00E9 informace o jejich chov\u00E1n\u00ED na elektricky nabit\u00FDch povr\u0161\u00EDch."@cs . "Navrhovan\u00FD grantov\u00FD projekt se zab\u00FDv\u00E1 strukturou, stanoven\u00EDm a interakcemi oligodeoxynukleotid\u016F v roztoku a na povr\u0161\u00EDch. Jeho \u0159e\u0161en\u00EDm budou z\u00EDsk\u00E1ny nov\u00E9 informace o oligonukleotidech a nukleov\u00FDch kyselin\u00E1ch na nabit\u00FDch f\u00E1zov\u00FDch rozhran\u00EDch realizovan\u00FDch pevn\u00FDmi a modifikovan\u00FDmi elektrodami. Navrhovan\u00E9 c\u00EDle budou spln\u011Bny s vyu\u017Eit\u00EDm voltametrick\u00FDch a chronopotenciometrick\u00FDch metod a jejich kombinac\u00ED s nov\u00FDmi technikami, jako je proudov\u00E1 eliminace a adsorptivn\u00ED rozpou\u0161tec\u00ED p\u0159enos. O\u010Dek\u00E1v\u00E1me, \u017Ee z\u00EDskan\u00E9 analytick\u00E9 metodiky budou vyu\u017Eity ve v\u00FDvoji jednoduch\u00FDch, citliv\u00FDch a selektivn\u00EDch biosenzor\u016F pro elektrochemickou detekci hybridizaci DNA. Vysoce citliv\u00E9 metody stanoven\u00ED b\u00E1z\u00ED nukleov\u00FDch kyselin naleznou vyu\u017Eit\u00ED i v r\u016Fzn\u00FDch oblastech biochemie a molekul\u00E1rn\u00ED biologie." . "IAA100040602" . "2006-01-01+01:00"^^ . "2008-12-31+01:00"^^ . "2009-07-02+02:00"^^ . "The proposed research is dealing with structure, determination and interactions of oligodeoxynucleotides in solution and at surfaces. New important information should be obtained about oligonucleotides and nucleic acids at electrically charged surfaces realized by solid and modifies electrodes. Proposal goals will be achieved using voltammetric and chronopotentiometric methods and their combination with new techniques such as current elimination and adsorptive transfer stripping. In addition, it is expected that analytical procedures obtained in this project will be utilized in development of simple, sensitive and selective biosensor for the electrochemical detection of DNA hybridisation. Highly sensitive methods based on the detection of nucleic acid bases will be developed to find application in various fields of biochemistry and molecular biology."@en . "Nov\u00E9 p\u0159\u00EDstupy v elektrochemick\u00E9 anal\u00FDze nukleov\u00FDch kyselin a oligonukleotid\u016F zam\u011B\u0159en\u00E9 na ultrasensitivn\u00ED mikrodetekci DNA a detekci DNA hybridizace." . " purine derivatives" . " voltammetry" . "The proposal was dealing with the analysis of oligonucleotides and their components by adsorptive stripping voltammetry in combination with elimination voltammetry aimed at finding new information about their behavior at electrically charged surfaces."@en . .