"CDC25 fosfat\u00E1zy hraj\u00ED d\u016Fle\u017Eitou roli v\u00A0aktivaci CDK a regulaci bun\u011B\u010Dn\u00E9ho cyklu. Lidsk\u00FD i my\u0161\u00ED genom k\u00F3duje t\u0159i CDC25 fosfat\u00E1zy: A, B a C. Zji\u0161t\u011Bn\u00ED z\u00A0lidsk\u00FDch n\u00E1dorov\u00FDch lini\u00ED ukazuj\u00ED, \u017Ee CDC25B je postradateln\u00E1 pro norm\u00E1ln\u00ED bun\u011B\u010Dn\u00FD cyklus, av\u0161ak \u017Ee je nezbytn\u00E1 pro n\u00E1vrat do bun\u011B\u010Dn\u00E9ho cyklu z\u00A0G2 f\u00E1ze po DNA po\u0161kozen\u00ED. Bylo publikov\u00E1no, \u017Ee Cdc25B deficientn\u00ED (KO) my\u0161i jsou \u017Eivotaschopn\u00E9, av\u0161ak sami\u010Dky neplodn\u00E9 z\u00A0d\u016Fvod\u016F neschopnosti oocyt\u016F znovuzah\u00E1jit mei\u00F3zu. Zda-li netransformovan\u00E9 prim\u00E1rn\u00ED bu\u0148ky z\u00A0KO my\u0161\u00ED maj\u00ED norm\u00E1ln\u00ED n\u00E1vrat do bun\u011B\u010Dn\u00E9ho cyklu nen\u00ED dosud zn\u00E1mo. Na\u0161e p\u0159edb\u011B\u017En\u00E9 v\u00FDsledky p\u0159ekvapiv\u011B ukazuj\u00ED, \u017Ee 5 \u2013 60% oocyt\u016F z\u00A0KO my\u0161\u00ED je schopno znovuzah\u00E1jen\u00ED mei\u00F3zy, by\u0165 se zpo\u017Ed\u011Bn\u00EDm. Tato zji\u0161t\u011Bn\u00ED vedou k\u00A0ot\u00E1zce, jestli je CDC25B fosfat\u00E1zov\u00E1 aktivita jak v\u00A0oocytech, tak somatick\u00FDch bu\u0148k\u00E1ch z KO my\u0161\u00ED efektivn\u011B vyru\u0161ena. Pokus\u00EDme se odpov\u011B\u010F na ot\u00E1zky zda-li je CDC25B skute\u010Dn\u011B pot\u0159eba pro 1/ znovuzah\u00E1jen\u00ED mei\u00F3zy, 2/ n\u00E1vrat do bun\u011B\u010Dn\u00E9ho cyklu; a 3/ norm\u00E1ln\u00ED mit\u00F3zu. Studie budou provedeny jak na oocytech, tak prim\u00E1rn\u00EDch somatick\u00FDch a dosp\u011Bl\u00FDch kmenov\u00FDch bu\u0148k\u00E1ch z\u00A0KO my\u0161\u00ED. Pou\u017Eit\u00ED RNAi n\u00E1m umo\u017En\u00ED ov\u011B\u0159it \u00FA\u010Dinnost vy\u0159azen\u00ED CDC25B v\u00A0KO my\u0161\u00EDch. Znovuzah\u00E1jen\u00ED mei\u00F3zy a n\u00E1vrat do bun\u011B\u010Dn\u00E9ho cyklu sd\u00EDl\u00ED mnoh\u00E9 bun\u011B\u010Dn\u00E9 signalizace, proto se zam\u011B\u0159\u00EDme na porovn\u00E1n\u00ED t\u011Bchto proces\u016F z\u00A0pohledu CDC25B." . . . "Role CDC25B fosfat\u00E1zy p\u0159i n\u00E1vratu do bun\u011B\u010Dn\u00E9ho cyklu: Srovn\u00E1n\u00ED znovuzah\u00E1jen\u00ED mei\u00F3zy u oocyt\u016F a zotaven\u00ED po G2 kontroln\u00EDm bodu u somatick\u00FDch bun\u011Bk" . . "GPP301/11/P081" . . "0"^^ . . . . . . "CDC25B bun\u011B\u010Dn\u00FD cyklus mit\u00F3ta mei\u00F3za G2 kontroln\u00ED bod knock out my\u0161i n\u00E1vrat do bun\u011B\u010Dn\u00E9ho cyklu"@en . "2014-06-25+02:00"^^ . "CDC25 phosphatases play crucial role in CDK activation and regulation of cell cycle progression. Human and mouse genomes contain three CDC25s: A, B and C. Data from human cancerous cells suggest that CDC25B is not essential for normal cell cycle progression, but it is essential for G2 checkpoint recovery (cell cycle resumption). The published work demonstrates that Cdc25B deficient (KO) mice are viable but female are infertile due to inability to resume meiosis. Whether nontransformed somatic cells from KO mice have normal G2 checkpoint recovery is unknown. Surprisingly our preliminary data show that 5 up to 60% of oocytes from KO mice are able to resume meiosis despite the significant delay. This raises the question if CDC25B phosphatase activity disruption was effective in both somatic cells and oocytes from these KO mice. We will attempt to answer questions whether CDC25B is essential for 1/resumption of meiosis, 2/G2 checkpoint recovery, and 3/ normal mitotic progression. Fully grown oocytes and primary somatic cells including adult stem cells from KO mice will be used. RNAi approaches allow us to verify Cdc25B gene targeting. Resumption of meiosis and G2 checkpoint recovery share many common aspects. We will compare these processes from the view of CDC25B signaling."@en . "2011-01-01+01:00"^^ . . "Projekt vyprodukoval c\u00EDlen\u011B 2 publikace (IF 4-6), jedna dal\u0161\u00ED je je\u0161t\u011B v recenzn\u00EDm \u0159\u00EDzen\u00ED. \u0158e\u0161itel je na v\u0161ech \u010Dl\u00E1nc\u00EDch, na dvou 1. a koresponduj\u00EDc\u00ED, co\u017E ukazuje na jeho budouc\u00ED seniorn\u00ED roli. Byl pou\u017Eit mutantni\u0301 kmen mys\u030Ci\u0301 s inaktivaci\u0301 genu pro Cdc25b, je\u017E je nezbytna pro plnou aktivaci Aurora kina\u0301zy A a pro biogenezi MTOC (ekvivalent centrosomu) v oocytech."@cs . . "Project produced 2 focused publications (IF 4-6), 1 other under-revision - Investigator is part of them (In two is a corresponding author). Project utilized transgenic murine strain of Cdc25b and provided evidence of its need during Aurora kinase A activation in oocytes. Postdoctoral project appears to be very succesful and helped to transform the investigator into an independent scientist."@en . . "Role of CDC25B phosphatase during cell cycle resumption: The Comparison of meiotic resumption in oocytes and G2 checkpoint recovery in somatic cells"@en . . . . . "3"^^ . "0"^^ . "3"^^ . "2013-12-31+01:00"^^ . . . . "1"^^ . "2013-06-12+02:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GPP301/11/P081"^^ .